Effect of 532nm photodynamic therapy with hemoporfin on the expression of vascular endothelial growth factor in cultured human vascular endothelial cells

Wenxin Dong; Xuting Zhang; Zhong Lu

doi:10.1016/j.pdpdt.2020.101793

Effect of 532nm photodynamic therapy with hemoporfin on the expression of vascular endothelial growth factor in cultured human vascular endothelial cells

Photodiagnosis Photodyn Ther. 2020 Jun:30:101793. doi: 10.1016/j.pdpdt.2020.101793. Epub 2020 Apr 26.

Authors

Wenxin Dong¹, Xuting Zhang², Zhong Lu³

Affiliations

¹ Department of Dermatology, Huashan Hospital Fudan University, Shanghai, China; Department of Medical Cosmetic Center, Shanghai Skin Disease Hospital, Shanghai, China.
² Department of Dermatology, Huashan Hospital Fudan University, Shanghai, China; Department of Dermatology, Shanghai Sixth People's Hospital, Shanghai, China.
³ Department of Dermatology, Huashan Hospital Fudan University, Shanghai, China. Electronic address: luzhong20100806@qq.com.

PMID: 32344194
DOI: 10.1016/j.pdpdt.2020.101793

Abstract

Background: Photodynamic therapy (PDT) with hemoporfin (hematoporphyrinmonomethyl ether, HMME) and 532 nm continuous-wave lasers is effective for port-wine stains (PWS) and is considered as a promising treatment modality. The vascular endothelial growth factor (VEGF) is involved in the development of PWS. This study aimed to investigate the effect of 532 nm-HMME-PDT on the in vitro expression of VEGF in human umbilical vein endothelial cells (HUVECs) exposed to different power levels of 532 nm laser and different concentrations of HMME.

Method: The CCK-8 assay was performed to assess cell viability. Enzyme-linked immunosorbent assay (ELISA) was performed to measure VEGF secretion. VEGF and VEGF receptor (VEGFR) mRNA expression levels were detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR).

Results: Within 72 h after HMME-PDT, cell viability was inhibited, secretion and expression of VEGF was decreased, and expression of VEGFR mRNA was significantly decreased with the increase of HMME concentration.

Conclusion: 532 nm-HMME-PDT can downregulate the expression of VEGF and VEGFR mRNA. Future studies are necessary to examine the HMME doses to achieve better efficacy with fewer adverse effects.

Keywords: Hemoporfin; Photodynamic therapy; Port-wine stains; Vascular endothelial growth factor.

MeSH terms

Cell Survival
Dose-Response Relationship, Drug
Enzyme-Linked Immunosorbent Assay
Hematoporphyrins / pharmacology*
Human Umbilical Vein Endothelial Cells
Humans
Lasers, Dye / therapeutic use*
Low-Level Light Therapy / methods*
Photochemotherapy / methods*
RNA, Messenger
Real-Time Polymerase Chain Reaction
Receptors, Vascular Endothelial Growth Factor / biosynthesis
Vascular Endothelial Growth Factors / biosynthesis*

Substances

Hematoporphyrins
RNA, Messenger
Vascular Endothelial Growth Factors
hematoporphyrin monomethyl ether
Receptors, Vascular Endothelial Growth Factor