Multifunctional laser speckle imaging

E Du; Shuhao Shen; Shau Poh Chong; Nanguang Chen

doi:10.1364/BOE.388856

Multifunctional laser speckle imaging

Biomed Opt Express. 2020 Mar 13;11(4):2007-2016. doi: 10.1364/BOE.388856. eCollection 2020 Apr 1.

Authors

E Du¹, Shuhao Shen¹, Shau Poh Chong¹, Nanguang Chen¹

Affiliation

¹ Optical Bioimaging Laboratory, Department of Biomedical Engineering, National University of Singapore, 7 Engineering Drive 1, Singapore 117574, Singapore.

Abstract

We have developed a multi-functional laser speckle imaging system, which can be operated in both the surface illumination laser speckle contrast imaging (SI-LSCI) mode and the line scan laser speckle contrast imaging (LS-LSCI) mode. The system has been applied to imaging the chicken embryos to visualize both the blood flow and morphological details of the vasculature. The experimental results demonstrated that LS-LSCI is capable of detecting and quantifying blood flow in blood vessels smaller and deeper than those detectable by conventional SI-LSCI. Furthermore, the line scan mode is also capable of producing depth-resolved absorption-based morphological images of tissue, augmenting flow-based functional images.