Integrated effects of fractional laser microablation and sonophoresis on skin immersion optical clearing in vivo

Elina A Genina; Alexey N Bashkatov; Georgy S Terentyuk; Valery V Tuchin

doi:10.1002/jbio.202000101

Integrated effects of fractional laser microablation and sonophoresis on skin immersion optical clearing in vivo

J Biophotonics. 2020 Jul;13(7):e202000101. doi: 10.1002/jbio.202000101. Epub 2020 May 8.

Authors

Elina A Genina^{1

2}, Alexey N Bashkatov^{1

2}, Georgy S Terentyuk^{3

4

5}, Valery V Tuchin^{1

2

6}

Affiliations

¹ Department of Optics and Biophotonics, Saratov State University, Saratov, Russia.
² Interdisciplinary Laboratory of Biophotonics, Tomsk State University, Tomsk, Russia.
³ Research Institute of Fundamental and Clinical Uronephrology, Saratov State Medical University, Saratov, Russia.
⁴ The First Veterinary Clinics, Saratov, Russia.
⁵ Research Department, Saratov State University, Saratov, Russia.
⁶ Laboratory of Laser Diagnostics of Technical and Living Systems, Institute of Precision Mechanics and Control of the RAS, Saratov, Russia.

PMID: 32339439
DOI: 10.1002/jbio.202000101

Abstract

This study is aimed to find an approach for effective skin optical clearing in vivo using polyethylene glycol 300 (PEG-300) as an optical clearing agent in combination with physical enhancers: fractional laser microablation (FLMA) and/or low-frequency sonophoresis. In this study albino outbred rats were used. Light attenuation coefficient and optical clearing potential (OCP) of these approaches were evaluated in upper (from ~70 to ~200 μm) and middle (from ~200 to ~400 μm) dermis separately using optical coherence tomography. In 30 minutes, OCP of sonophoresis in combination with FLMA and PEG-300 in the upper dermis was the maximal (2.3 ± 0.4) in comparison with other treatments in this time point. The most effective approach for optical clearing of middle dermis was PEG-300 and sonophoresis; but the maximal value of OCP (1.6 ± 0.1) was achieved only in 90 minutes.

Keywords: immersion; laser tissue ablation; optical coherence tomography; skin; ultrasonic therapy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Immersion*
Lasers
Light
Rats
Skin*
Tomography, Optical Coherence