Efficient CRISPR/Cas9-based plant genomic fragment deletions by microhomology-mediated end joining

Jiantao Tan; Yanchang Zhao; Bin Wang; Yu Hao; Yaxi Wang; Yangyang Li; Wanni Luo; Wubei Zong; Gousi Li; Shuifu Chen; Kun Ma; Xianrong Xie; Letian Chen; Yao-Guang Liu; Qinlong Zhu

doi:10.1111/pbi.13390

Efficient CRISPR/Cas9-based plant genomic fragment deletions by microhomology-mediated end joining

Plant Biotechnol J. 2020 Nov;18(11):2161-2163. doi: 10.1111/pbi.13390. Epub 2020 May 22.

Authors

Jiantao Tan^{1

2

3}, Yanchang Zhao^{1

3}, Bin Wang^{1

3}, Yu Hao^{1

3}, Yaxi Wang^{1

3}, Yangyang Li^{1

4}, Wanni Luo^{1

3}, Wubei Zong^{1

3}, Gousi Li^{1

3}, Shuifu Chen^{1

3}, Kun Ma^{1

3}, Xianrong Xie^{1

2

3}, Letian Chen^{1

2

3}, Yao-Guang Liu^{1

2

3}, Qinlong Zhu^{1

2

3}

Affiliations

¹ State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou, China.
² Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China.
³ College of Life Sciences, South China Agricultural University, Guangzhou, China.
⁴ Chongqing Engineering Research Center for Floriculture, College of Horticulture and Landscape, Southwest University, Chongqing, China.

No abstract available

Keywords: CRISPR/Cas9; MMEJ; NHEJ; genome editing; genomic fragment deletion.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems* / genetics
Clustered Regularly Interspaced Short Palindromic Repeats*
DNA End-Joining Repair
Gene Editing
Genomics