Novel betaine-amino acid based natural deep eutectic solvents for enhancing the enzymatic hydrolysis of corncob

Yuan Liang; Wenjing Duan; Xiaoxi An; Yingyun Qiao; Yuanyu Tian; Haifeng Zhou

doi:10.1016/j.biortech.2020.123389

Novel betaine-amino acid based natural deep eutectic solvents for enhancing the enzymatic hydrolysis of corncob

Bioresour Technol. 2020 Aug:310:123389. doi: 10.1016/j.biortech.2020.123389. Epub 2020 Apr 18.

Authors

Yuan Liang¹, Wenjing Duan¹, Xiaoxi An¹, Yingyun Qiao², Yuanyu Tian³, Haifeng Zhou⁴

Affiliations

¹ Key Laboratory of Low Carbon Energy and Chemical Engineering, College of Chemistry and Bioengineering, Shandong University of Science and Technology, Qingdao 277590, China.
² State Key Laboratory of Heavy Oil Processing, China University of Petroleum (East China), Qingdao 266580, China.
³ Key Laboratory of Low Carbon Energy and Chemical Engineering, College of Chemistry and Bioengineering, Shandong University of Science and Technology, Qingdao 277590, China; State Key Laboratory of Heavy Oil Processing, China University of Petroleum (East China), Qingdao 266580, China.
⁴ Key Laboratory of Low Carbon Energy and Chemical Engineering, College of Chemistry and Bioengineering, Shandong University of Science and Technology, Qingdao 277590, China. Electronic address: zhouhaifeng-qd@163.com.

PMID: 32335347
DOI: 10.1016/j.biortech.2020.123389

Abstract

A novel natural deep eutectic solvent (NDES) with water content ranging from 65 to 93 wt%, in which betaine (Bet) acts as the cation and amino acids (AAs) as the anions, was prepared by a simple and green chemical route. [Bet][AA] NDES showed excellent xylan and lignin solubility, however, scare cellulose solubility. A mild and facile pretreatment process with [Bet][AA] NDES was carried out at 60 °C for 5 h. The enzymatic hydrolysis efficiency of cellulose and corncob was significantly improved. Detailed characterization showed that the enhancement of cellulose digestibility derived mainly from xylan and lignin removal. Xylan and lignin removal for [Bet][Lys]-W87 was 47.68 and 49.06%, while it was 42.20% and 57.01% for [Bet][Arg]-W82, respectively. FT-IR, SEM, XRD, and HSQC NMR studies confirmed the effectiveness and mechanism of [Bet][Lys]-W87 and [Bet][Arg]-W82 on biomass pretreatment.

Keywords: Betaine amino acids; Enzymatic hydrolysis; Natural deep eutectic solvent; Pretreatment.

MeSH terms

Amino Acids
Betaine*
Biomass
Hydrolysis
Lignin
Solvents
Spectroscopy, Fourier Transform Infrared
Zea mays*

Substances

Amino Acids
Solvents
Betaine
Lignin