Mixing strategies - Activated carbon nexus: Rapid start-up of thermophilic anaerobic digestion with the mesophilic anaerobic sludge as inoculum

Abstract

This study evaluated the mixing - activate carbon nexus in anaerobic digestion with the aim of accelerating start-up of thermophilic anaerobic co-digestion of food waste and chicken manure using mesophilic anaerobic sludge as inoculum. Results showed that the methane yield in the continuous stirred reactor is 71.3% higher than that of intermittent agitated reactor, and the addition of activated carbon can further improve the yield of methane by 18.2%. Continuous mixing mode followed by intermittent mixing was proved to be an alternative strategy to accelerate start-up of thermophilic anaerobic digestion. The optimum mixing time of 120 s/hour were obtained using computational fluid dynamics modeling. Analysis of genomic annotation metabolism indicated that the addition of activated carbon enhanced the dominant metabolism pathways of amino acid, methane and energy. Results of enzymes gene expression suggested that carbohydrates esterases, glycoside hydrolases and glycosyl transferases were dominant, respectively.

Keywords: Activated carbon; Anaerobic digestion; Food waste; Mixing; Thermophilic.