Identification and characterization of novel antioxidant peptides from mackerel (Scomber japonicus) muscle protein hydrolysates

Khawaja Muhammad Imran Bashir; Jae Hak Sohn; Jin-Soo Kim; Jae-Suk Choi

doi:10.1016/j.foodchem.2020.126809

Identification and characterization of novel antioxidant peptides from mackerel (Scomber japonicus) muscle protein hydrolysates

Food Chem. 2020 Apr 15:323:126809. doi: 10.1016/j.foodchem.2020.126809. Online ahead of print.

Authors

Khawaja Muhammad Imran Bashir¹, Jae Hak Sohn², Jin-Soo Kim³, Jae-Suk Choi⁴

Affiliations

¹ German Engineering Research Center for Life Science Technologies in Medicine and Environment, 31, Gwahaksan 1-ro, 60 Bean-gil, Gangseo-gu, Busan 46742, Republic of Korea; Seafood Research Center, IACF, Silla University, 606, Advanced Seafood Processing Complex, Wonyang-ro, Amnam-dong, Seo-gu, Busan 49277, Republic of Korea. Electronic address: imranagrarian3@gmail.com.
² Seafood Research Center, IACF, Silla University, 606, Advanced Seafood Processing Complex, Wonyang-ro, Amnam-dong, Seo-gu, Busan 49277, Republic of Korea; Department of Food Biotechnology, Division of Bioindustry, College of Medical and Life Sciences, Silla University, 140, Baegyang-daero 700 beon-gil, Sasang-gu, Busan 46958, Republic of Korea. Electronic address: jhsohn@silla.ac.kr.
³ Department of Seafood and Aquaculture Science, Gyeongsang National University, 38 Cheondaegukchi-gil, Tongyeong-si, Gyeongsangnam-do 53064, Republic of Korea. Electronic address: jinsukim@gnu.ac.kr.
⁴ Seafood Research Center, IACF, Silla University, 606, Advanced Seafood Processing Complex, Wonyang-ro, Amnam-dong, Seo-gu, Busan 49277, Republic of Korea; Department of Food Biotechnology, Division of Bioindustry, College of Medical and Life Sciences, Silla University, 140, Baegyang-daero 700 beon-gil, Sasang-gu, Busan 46958, Republic of Korea. Electronic address: jsc1008@silla.ac.kr.

PMID: 32330643
DOI: 10.1016/j.foodchem.2020.126809

Abstract

Antioxidant peptides are commonly used as functional ingredient in the pharmaceutical industries. Here, we characterized the antioxidant peptides from mackerel muscle protein hydrolysates (MPHs). MPHs showing higher bioactivities were separated into seven groups by FPLC. MPH-3 which exhibited significantly higher (p < 0.05) DPPH scavenging activity (32.12 ± 3.01%) was fractionated using RP-HPLC to obtain purified fractions A and B, which were further subjected to MALDI-TOF/TOF-MS for mass fingerprinting. Fraction A exhibited the highest (p < 0.05) DPPH scavenging activity (34.11 ± 1.52%), and it contained 21 peptides characterized by LC-MS/MS-. Ten peptides were synthesized, and their antioxidant activities were evaluated; one of the peptides, ALSTWTLQLGSTSFSASPM, showed the highest (p < 0.05) DPPH scavenging activity (36.34 ± 4.64%) and another peptide, LGTLLFIAIPI, exhibited the highest (p < 0.05) SOD-like activity (28.94 ± 4.19%). The results of this study indicate that MPHs could serve as a suitable source of antioxidant peptides.

Keywords: Amino acid sequencing; Bioactive peptide; Enzymatic hydrolysis; Muscle protein hydrolysate; Tandem mass spectrometry.