Isolation and characterization of bovine herpes virus 5 (BoHV5) from cattle in India

PLoS One. 2020 Apr 24;15(4):e0232093. doi: 10.1371/journal.pone.0232093. eCollection 2020.

Abstract

Bovine herpesvirus 1 (BoHV1) and 5 (BoHV5) are genetically and antigenically related alphaherpesviruses. Infection with one virus induces protective immunity against the other. However, disease associated with BoHV1 and BoHV5 varies significantly; whereas BoHV1 infection is usually associated with rhinotracheitis and abortion, BoHV5 causes encephalitis in cattle. BoHV5 outbreaks are sporadic and mainly restricted to the South American countries. We report BoHV5 infection for the first time from aborted cattle in India. Based on the characteristic cytopathic effects in MDBK cells, amplification of the viral genome by PCR, differential PCR for BoHV1/BoHV5, nucleotide sequencing and restriction endonuclease patterns, identity of the virus was confirmed as BoHV5 subtype A. Serum samples from the aborted cattle strongly neutralized both BoHV1 and BoHV5 suggesting an active viral infection in the herd. Upon UL27, UL44 and UL54 gene-based sequence and phylogenetic analysis, the isolated virus clustered with BoHV5 strains and showed highest similarity with the Brazilian BoHV5 strains.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alphaherpesvirinae / genetics
  • Animals
  • Antibodies, Viral / blood
  • Cattle
  • Cattle Diseases / epidemiology
  • Disease Outbreaks / veterinary
  • Genome, Viral / genetics
  • Herpesviridae Infections / virology
  • Herpesvirus 1, Bovine / genetics
  • Herpesvirus 1, Bovine / metabolism
  • Herpesvirus 5, Bovine / genetics*
  • Herpesvirus 5, Bovine / isolation & purification*
  • Herpesvirus 5, Bovine / metabolism*
  • India
  • Phylogeny

Substances

  • Antibodies, Viral

Grants and funding

This work was supported by the Science and Engineering Research Board, Department of Science and Technology, Government of India, Grant number CRG/2018/004747 to N. Kumar and Indian Council of Agricultural Research, Grant Number IXX11882 to N. Kumar and SB. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.