Abstract
Three new diterpenoids (a cephalotane, an abietane and a 9(10→20)-abeo-abietane) and one new flavonoid, together with 11 known compounds, were isolated from the twigs of Cephalotaxus fortunei var. alpina. The new compounds were identified by comprehensive spectroscopic (including 1D and 2D-NMR and HR-ESI-MS) analysis. Anti-inflammatory, immunosuppressive and cytotoxic activities of three new compounds were evaluated. 3β,20-epoxyabieta-8,11,13-triene-3α,12-diol showed weak cytotoxicity against tumor cell lines NCI-H1975, HepG2, MCF-7, while fortalpinoid R and 3-acetonyl-3,5,7,4'-tetrahydroxy-2-methoxyflavanone were not active at 80 μM. None of these compounds showed anti-inflammatory and immunosuppressive activities.
Keywords:
Cephalotaxus fortunei var alpina; cytotoxicity; diterpenoids; flavonoids.
© 2020 Wiley-VHCA AG, Zurich, Switzerland.
MeSH terms
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Animals
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Antineoplastic Agents, Phytogenic / chemistry*
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Antineoplastic Agents, Phytogenic / isolation & purification
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Antineoplastic Agents, Phytogenic / pharmacology
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Cell Line, Tumor
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Cell Survival / drug effects
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Cephalotaxus / chemistry*
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Cephalotaxus / metabolism
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Diterpenes / chemistry*
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Diterpenes / isolation & purification
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Diterpenes / pharmacology
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Flavonoids / chemistry*
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Flavonoids / isolation & purification
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Flavonoids / pharmacology
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Humans
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Macrophages / cytology
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Macrophages / drug effects
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Macrophages / metabolism
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Magnetic Resonance Spectroscopy
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Mice
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Molecular Conformation
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Plant Extracts / chemistry
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Plant Leaves / chemistry
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Plant Leaves / metabolism
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RAW 264.7 Cells
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Spectrometry, Mass, Electrospray Ionization
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Tumor Necrosis Factor-alpha / metabolism
Substances
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Antineoplastic Agents, Phytogenic
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Diterpenes
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Flavonoids
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Plant Extracts
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Tumor Necrosis Factor-alpha