Interaction of Cyclooxygenase-2 with Helicobacter pylori Induces Gastric Chronic Nonresolving Inflammation and the Formation of Syndrome of Internal Block of Static Blood in Helicobacter pylori-Related Gastric Diseases

Yun-Kai Dai; Yun-Zhan Zhang; Dan-Yan Li; Xu Chen; Lin Gong; Qi Luo; Shao-Yang Lan; Bin Chen; Jian-Yu Wu; Zi-Jing Zhang; Meng-Xin Huang; Jin-Tong Ye; Wei-Jing Chen; Ru-Liu Li; Ling Hu

doi:10.1155/2020/7340814

Interaction of Cyclooxygenase-2 with Helicobacter pylori Induces Gastric Chronic Nonresolving Inflammation and the Formation of Syndrome of Internal Block of Static Blood in Helicobacter pylori-Related Gastric Diseases

Evid Based Complement Alternat Med. 2020 Apr 6:2020:7340814. doi: 10.1155/2020/7340814. eCollection 2020.

Authors

Yun-Kai Dai¹, Yun-Zhan Zhang¹, Dan-Yan Li¹, Xu Chen^{1

2}, Lin Gong¹, Qi Luo², Shao-Yang Lan², Bin Chen², Jian-Yu Wu², Zi-Jing Zhang², Meng-Xin Huang¹, Jin-Tong Ye¹, Wei-Jing Chen¹, Ru-Liu Li¹, Ling Hu¹

Affiliations

¹ Institute of Gastroenterology, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
² First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.

Abstract

Cyclooxygenase-2 (COX-2) is an inducible enzyme stimulated by various inflammatory factors (IFs). Chronic gastritis is a classic model of "inflammation-cancer transformation" and Helicobacter pylori-related gastric diseases (HPGD) are specific ones of this model. Traditional Chinese Medicine (TCM) syndromes could play a predictive role in gastric histopathological evolution. To search for early warning evidence about "inflammation-cancer transformation," this study is about to explore interaction of COX-2 with Helicobacter pylori (Hp) in HPGD with different TCM syndromes. All included subjects underwent endoscopy and biopsy. Hp infection was detected by rapid urease test and methylene blue staining. Histopathological characteristics and COX-2 expression in gastric mucosa (GM) were, respectively, observed by hematoxylin-eosin and Elivision™ plus. SPSS 18.0 and Stata 11.0 statistical software packages were used for statistical analysis. Results of immunohistochemical staining in this study showed COX-2 expression in Hp-positive patients was stronger than that in Hp-negative ones. Spearman' analysis indicated that degrees of both Hp infection and COX-2 expression were positively correlated with those of gastric inflammation and inflammatory activity. Compared with the relative normal group, both severe dysplasia group and gastric carcinoma group had more severe Hp infection and COX-2 expression. Compared with the nonsyndrome, syndrome of internal block of static blood (IBSB) had higher scores in semiquantitative analysis of COX-2 protein expression among TCM groups. Moreover, multivariate logistics regression analysis suggested that patients with Hp infection could increase the risk of IBSB. These results indicated that COX-2 interacting with Hp could play an important role in transforming gastric chronic nonresolving inflammation into carcinoma in subjects with HPGD, as well as inducing the formation of IBSB. HPGD together with IBSB could be an early warning evidence for GM with histopathological evolution from benign to malignant.