Genome Sequence of Human Cytomegalovirus Ig-KG-H2, a Variant of Strain KG Propagated in the Presence of Neutralizing Antibodies

Ahmed Al Qaffas; Salvatore Camiolo; Jenna Nichols; Andrew J Davison; Amine Ourahmane; Xiaohong Cui; Mark R Schleiss; Laura Hertel; Dirk P Dittmer; Michael A McVoy

doi:10.1128/MRA.00063-20

Genome Sequence of Human Cytomegalovirus Ig-KG-H2, a Variant of Strain KG Propagated in the Presence of Neutralizing Antibodies

Microbiol Resour Announc. 2020 Apr 23;9(17):e00063-20. doi: 10.1128/MRA.00063-20.

Authors

Affiliations

¹ Department of Pediatrics, Virginia Commonwealth University, Richmond, Virginia, USA.
² MRC-University of Glasgow Centre for Virus Research, Glasgow, United Kingdom.
³ Center for Infectious Diseases and Microbiology Translational Research, Division of Pediatric Infectious Diseases, Minneapolis, Minnesota, USA.
⁴ Department of Pediatrics, School of Medicine, University of California, San Francisco, Oakland, California, USA.
⁵ Lineberger Comprehensive Cancer Center Program in Global Oncology, Department of Microbiology and Immunology, Center for AIDS Research, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
⁶ Department of Pediatrics, Virginia Commonwealth University, Richmond, Virginia, USA michael.mcvoy@vcuhealth.org.

Abstract

Human cytomegalovirus shed in infant urine was isolated and serially passaged in fibroblasts in the presence or absence of neutralizing antibodies. Comparison of the genome sequences of representative viruses Ig-KG-H2 (passed with antibody) and ϕ-KG-B5 (passed without antibody) revealed the presence of several mutations in each virus.

Abstract

Grants and funding