Synergistic interaction of diclofenac and its metabolites with selected antibiotics and amygdalin in wastewaters

Marzena Matejczyk; Piotr Ofman; Katarzyna Dąbrowska; Renata Świsłocka; Włodzimierz Lewandowski

doi:10.1016/j.envres.2020.109511

Synergistic interaction of diclofenac and its metabolites with selected antibiotics and amygdalin in wastewaters

Environ Res. 2020 Jul:186:109511. doi: 10.1016/j.envres.2020.109511. Epub 2020 Apr 14.

Authors

Marzena Matejczyk¹, Piotr Ofman², Katarzyna Dąbrowska³, Renata Świsłocka⁴, Włodzimierz Lewandowski⁴

Affiliations

¹ Bialystok University of Technology, Faculty of Civil Engineering and Environmental Sciences, Department of Chemistry, Biology and Biotechnology, Wiejska 45E Street, 15-351, Bialystok, Poland. Electronic address: m.matejczyk@pb.edu.pl.
² Bialystok University of Technology, Department of Environmental Engineering Technology, Bialystok University of Technology, Bialystok, 15-341, Poland.
³ Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology, Department of Microbiology, 36 Rakowiecka Str., 02-532, Warsaw, Poland.
⁴ Bialystok University of Technology, Faculty of Civil Engineering and Environmental Sciences, Department of Chemistry, Biology and Biotechnology, Wiejska 45E Street, 15-351, Bialystok, Poland.

PMID: 32325296
DOI: 10.1016/j.envres.2020.109511

Abstract

Diclofenac (DCF), a non-steroidal anti-inflammatory drug (NSAID) belongs to one of the most frequently detected pharmaceutical residues in the environment. Little is known on the interactions of DCF as well as its major biodegradation metabolites 4'-OHDCF and 5-OHDCF with chemical compounds found in wastewater, including antibiotics such as ampicillin and kanamycin. In the present work we examined the potential interactions between DCF, its metabolites 4'-OHDCF and 5-OHDCF and ampicyllin and kanamycin. We also measured the effect of the mixture of DCF with natural compound - amygdalin. We evaluated the following parameters: E. coli K-12 cells viability, growth inhibition of E. coli K-12 culture, genotoxicity, oxidative stress parameters: sodA promoter induction and ROS generation. The reactivity of E. coli SM recA:luxCDABE biosensor strain in wastewaters matrices contaminated with DCF and kanamycin was also monitored. Obtained results indicated that used antibiotics (ampicyllin, kanamycin) enhanced the toxic effect of DCF used individually and in the mixtures with its metabolites 4'-OHDCF and 5-OHDCF toward E. coli. Similar effect was also obtained in genotoxicity assay. The oxidative stress assays revealed that the highest level of ROS generation and sodA promoter induction were obtained also for the mixtures of DCF, its metabolites with antibiotics. It was also showed that amygdalin influenced the activity of DCF and its biodegradation metabolites. The strongest luminescence response of E. coli SM biosensor strain with recA:luxCDABE genetic construct in filtered treated wastewaters, comparable to control sample was noticed. Obtained results showed that DCF and its biodegradation metabolites 4'-OHDCF and 5-OHDCF can interact with tested antibiotics and compounds of natural origin, i.e. amygdalin to form mixtures showing stronger antimicrobial activity against E. coli than parent chemicals. Moreover the assays in wastewater matrices revealed that E. coli SM recA:luxCDABE biosensor strains is a good tool for bacteria monitoring in wastewater environments.

Keywords: Amygdalin; Antibiotics; Diclofenac; Microbial biosensors; Wastewaters.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amygdalin*
Anti-Bacterial Agents / toxicity
Diclofenac
Escherichia coli
Wastewater / analysis
Water Pollutants, Chemical* / analysis
Water Pollutants, Chemical* / toxicity

Substances

Anti-Bacterial Agents
Waste Water
Water Pollutants, Chemical
Diclofenac
Amygdalin