Cry80Aa1, a novel Bacillus thuringiensis toxin with mosquitocidal activity to Culex pipiens pallens

Yan Zhou; Zhongqi Wu; Jie Zhang; Yusong Wan; Wujun Jin; Youzhi Li; Xuanjun Fang

doi:10.1016/j.jip.2020.107386

Cry80Aa1, a novel Bacillus thuringiensis toxin with mosquitocidal activity to Culex pipiens pallens

J Invertebr Pathol. 2020 Jun:173:107386. doi: 10.1016/j.jip.2020.107386. Epub 2020 Apr 20.

Authors

Yan Zhou¹, Zhongqi Wu², Jie Zhang³, Yusong Wan⁴, Wujun Jin⁴, Youzhi Li¹, Xuanjun Fang⁵

Affiliations

¹ College of Life Science and Technology/State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530004, China(2).
² Institute of Life Science, Jiyang College of Zhejiang A&F University, Zhuji 311800, China(2); Hainan Institute of Tropical Agricultural Resources, Sanya 572025, China(2); Cuixi Academy of Biotechnology, Zhuji 311800, China.
³ Institute of Life Science, Jiyang College of Zhejiang A&F University, Zhuji 311800, China(2).
⁴ Biotechnology Research Institute of Chinese Academy of Agricultural Sciences, Beijing 100091, China.
⁵ College of Life Science and Technology/State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530004, China(2); Institute of Life Science, Jiyang College of Zhejiang A&F University, Zhuji 311800, China(2); Hainan Institute of Tropical Agricultural Resources, Sanya 572025, China(2); Cuixi Academy of Biotechnology, Zhuji 311800, China. Electronic address: jim.xj.fang@hitar.org.

PMID: 32325072
DOI: 10.1016/j.jip.2020.107386

Abstract

Bacillus thuringiensis (Bt) can synthesize insecticides to efficiently control insects. In this study, Bt strain S3580-1 with mosquitocidal activity was subjected to whole genome sequencing using an Illumina HiSeq 2000 system. A novel toxin, Cry80Aa1, was identified based on the resulting data. A conserved domain analysis revealed that Cry80Aa1 includes the Ricin_B_lectin domain (located at 10-150) and the Toxin_10 domain (located at 155-353). Phylogenetic tree analysis showed that Cry80Aa1 was in a distinct clade significantly distinguished from the known Cry proteins containing the Toxin_10 domain. Bioassays demonstrated that the Cry80Aa1 protein exhibited toxicity to third instar larvae of Culex pipiens pallens (LC₅₀: 71.9 μg/mL; 95% FL: 59.5-122.7 μg/mL).

Keywords: Bacillus thuringiensis; Cry80Aa1; Culex pipiens pallens; Toxin_10.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacillus thuringiensis Toxins*
Culex* / growth & development
Endotoxins*
Hemolysin Proteins*
Insecticides*
Larva / growth & development
Mosquito Control*
Pest Control, Biological*

Substances

Bacillus thuringiensis Toxins
Endotoxins
Hemolysin Proteins
Insecticides
insecticidal crystal protein, Bacillus Thuringiensis