Sustained monoculture often leads to the inhibition of plant growth, the decrease of the soil microbial diversity, and changes in soil microbial community composition, particularly to the accumulation of soil-borne pathogens. In this study, we conducted field experiments to investigate the practical effects of tilling the soil down to a depth of 40 cm (40dp) in combination with dazomet (D) soil fumigation and/or the application of a bio-organic fertilizer (B) on chrysanthemum growth, with a focus on the potential mechanisms underlying the responses of the soil microbiome. The growth indices of chrysanthemum were significantly (p < .05) increased in the DB + 40dp treatment compared to that in other treatments. The weighted and unweighted UniFrac distances in the principal coordinate analysis (PCoA) revealed that soil bacterial and fungal community compositions were separated according to the treatments. The abundance of genera potentially expressing growth promotion, such as Pseudomonas and Bacillus, was increased in the DB + 40dp treatment. In addition, the combined DB + 40dp treatment enhanced the activities of catalase, urease, sucrase, and β-d-glucosidase, and significantly increased the levels of available nitrogen, phosphorus, and potassium in the soil. The redundancy analysis (RDA) implied that the composition of the microbiome was correlated to soil enzymatic activities and soil potassium availability in the rhizosphere soil of chrysanthemum plants. Our findings suggest that the DB + 40dp treatment is a better strategy for improving chrysanthemum growth and regulating the rhizosphere microbiome in monoculture soils than the methods presently employed by commercial chrysanthemum producers.
Keywords: Illumina Hiseq; biofertilizer; microbial diversity; monoculture; soil microbiome.
© 2020 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.