Dataset on phenotypic characterization, on protein and genome analysis of three fluorescent Pseudomonas strains from mid-mountain water

Elodie Dussert; Mélissa Tourret; Barbara Deracinois; Matthieu Duban; Valérie Leclère; Benoit Cudennec; Rozenn Ravallec; Josette Behra-Miellet

doi:10.1016/j.dib.2020.105466

Dataset on phenotypic characterization, on protein and genome analysis of three fluorescent Pseudomonas strains from mid-mountain water

Data Brief. 2020 Apr 10:30:105466. doi: 10.1016/j.dib.2020.105466. eCollection 2020 Jun.

Authors

Elodie Dussert¹, Mélissa Tourret¹, Barbara Deracinois¹, Matthieu Duban¹, Valérie Leclère¹, Benoit Cudennec¹, Rozenn Ravallec¹, Josette Behra-Miellet¹

Affiliation

¹ Univ. Lille, INRA, ISA, Univ. Artois, Univ. Côte d'Opale, EA 7394 - ICV - Institut Charles Viollette, F-59000 Lille, France.

Abstract

The identification of non-fermentative Gram negative bacilli from run-off and spring water, including fluorescent Pseudomonas is very complex and investigations are needed to contribute to the systematic of these bacteria. In this dataset, the phenotypical profiles of three strains isolated from Vosges mountains first identified as Pseudomonas fluorescens were determined using API^Ⓡ 50 CH galleries. Then, the identification of their proteins released directly into water was carried out using tandem/mass spectrometry after separating proteins on native two-dimensional polyacrylamide gels. Finally, genotypic analysis data is presented, that illustrates biodiversity in this fluorescent bacterial group. This data is referred by a research article entitled "Fluorescent Pseudomonas strains from mid-mountain water able to release antioxidant proteins directly into water".

Keywords: ANI, average nucleotide identity; Bacterial identification; Fluorescent Pseudomonas; Genome sequencing; MALDI, matrix assisted laser desorption ionization; MS, mass spectrometry; MS/MS, tandem mass spectrometry; MW, molecular weight; NCBI, National Center for Biotechnology Information; NR, not reviewed; Nb, number of experimental peptides recognized; P., Pseudomonas; PFF, peptide fragment fingerprinting; PMF, peptide mass fingerprinting; Proteomics; R, reviewed; Spot no., spot number; TCS, tetra correlation search; TOF/TOF, time-of-flight/time-of-flight; ppm, parts per million.