High-speed single molecule imaging datasets of membrane proteins in rat basophilic leukemia cells

Hanieh Mazloom-Farsibaf; William K Kanagy; Diane S Lidke; Keith A Lidke

doi:10.1016/j.dib.2020.105424

High-speed single molecule imaging datasets of membrane proteins in rat basophilic leukemia cells

Data Brief. 2020 Apr 6:30:105424. doi: 10.1016/j.dib.2020.105424. eCollection 2020 Jun.

Authors

Hanieh Mazloom-Farsibaf¹, William K Kanagy², Diane S Lidke^{2

3}, Keith A Lidke^{1

3}

Affiliations

¹ Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM, United States.
² Department of Pathology, University of New Mexico, Albuquerque, NM, United States.
³ Comprehensive Cancer Center, University of New Mexico, Albuquerque, NM, United States.

Abstract

A high-speed fluorescence microscope operating at a 490 Hz frame rate was used to image two different membrane proteins- the high-affinity IgE receptor FcɛRI, a transmembrane protein, and an outer-leaflet GPI-anchored protein. The IgE receptor was imaged via IgE labeled with Janelia Fluor 646 and the GPI-anchored protein was imaged using a GPI-GFP fusion protein and an ATTO 647 N labeled anti-GFP nanobody. Data was collected for both proteins in untreated cells and cells that had actin stabilized by phalloidin. This dataset can be used for development and testing of single-particle tracking methods on experimental data and to explore the hypothesis that the actin cytoskeleton may affect the movement of membrane proteins.

Keywords: Actin; Fluorescence microscopy; Membrane proteins; Single-particle tracking.

Abstract

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