Genetic Engineering of Filamentous Fungi for Efficient Protein Expression and Secretion

Qin Wang; Chao Zhong; Han Xiao

doi:10.3389/fbioe.2020.00293

Genetic Engineering of Filamentous Fungi for Efficient Protein Expression and Secretion

Front Bioeng Biotechnol. 2020 Apr 8:8:293. doi: 10.3389/fbioe.2020.00293. eCollection 2020.

Authors

Qin Wang¹, Chao Zhong^{2

3}, Han Xiao¹

Affiliations

¹ State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, and Laboratory of Molecular Biochemical Engineering, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
² Materials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University, Shanghai, China.
³ Materials Synthetic Biology Center, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

Abstract

Filamentous fungi are considered as unique cell factories for protein production due to the high efficiency of protein secretion and superior capability of post-translational modifications. In this review, we firstly introduce the secretory pathway in filamentous fungi. We next summarize the current state-of-the-art works regarding how various genetic engineering strategies are applied for enhancing protein expression and secretion in filamentous fungi. Finally, in a future perspective, we discuss the great potential of genome engineering for further improving protein expression and secretion in filamentous fungi.

Keywords: expression; filamentous fungi; genetic engineering; genome engineering; protein secretion.

Publication types

Review