Objective: To investigate the differentiation of acute promyelocytic leukemia (APL) cells induced by Tetrandrine targeting MUC1.
Methods: HL-60 cells at logarithmic growth phase were choosen as object of reaserch and were divided into four groups: control, ATRA, Tetrandrine and ATRA+Tetrandrine group. Cell morphologic changes in each group were observed by microscopy. The change of cell differentiation ability was analyzed by nitro-blue tetrazolium (NBT) reduction test. The expression of cell surface differentiation antigen CD11b was measured by flow cytometry. The expression of MUC1 protein was detected by Western blot.
Results: The differentiation of HL-60 cell could be induced by Tetrandrine. The NBT reduction-positive rate of ATRA+Tetrandrine group was significantly higher than that in ATRA group and Tetrandrine group (P<0.05). The percentage of CD11b positive cells in ATRA+Tetrandrine group(43.62％±1.38％) was significantly higher than that in Tetrandrine group(15.25％±2.11％), ATRA group (28.84％±7.53％) and control group(8.16％±1.01％) (P<0.05). The content of MUC1 protein in Tetrandrine group was significantly lower than that in control group and ATRA group (P<0.05).
Conclusion: Tetrandrine and ATRA can synergize to promote the differentiation and maturation of HL-60 cells, and the mechanism may be related with MUC1 expression.
题目: 汉防己甲素诱导HL-60细胞分化.
目的: 探讨汉防己甲素(Tetrandrine)靶向黏蛋白1（MUC1）诱导急性早幼粒细胞白血病(APL)细胞分化.
方法: 以HL-60细胞株作为研究对象，选取对数生长期细胞，分为空白对照、ATRA、汉防己甲素和ATRA+汉防己甲素，共4组。显微镜下观察各组细胞形态的变化；用NBT还原实验分析细胞分化能力的变化；采用流式细胞术检测细胞表面分化抗原CD11b表达的变化；采用Western blot 检测MUC1表达的变化.
结果: 汉防己甲素可以诱导HL-60细胞分化； ATRA+汉防己甲素组NBT还原阳性率明显高于ATRA和汉防己甲素组（P＜0.05）；ATRA+汉防己甲素组CD11b+ 细胞百分率(43.62％±1.38％) 明显高于汉防己甲素(15.25％±2.11％)、ATRA(2184％±7.53％)和空白对照组(8.16％±1.01％)（P＜0.05）；汉防己甲素组MUC1蛋白量明显低于空白对照组和ATRA组（P＜0.05）.
结论: 汉防己甲素体外能协同ATRA使HL-60细胞分化成熟，其作用机制可能与调控MUC1的表达有关.