Cervical, anal and oral HPV detection and HPV type concordance among women referred for colposcopy

Maria Nasioutziki; Kimon Chatzistamatiou; Panagiotis-Dimitrios Loufopoulos; Eleftherios Vavoulidis; Nikolaos Tsampazis; George-Chrysostomos Pratilas; Anastasios Liberis; Vasiliki Karpa; Evanggelos Parcharidis; Angelos Daniilidis; Konstantinos Spanos; Konstantinos Dinas

doi:10.1186/s13027-020-00287-7

Cervical, anal and oral HPV detection and HPV type concordance among women referred for colposcopy

Infect Agent Cancer. 2020 Apr 15:15:22. doi: 10.1186/s13027-020-00287-7. eCollection 2020.

Affiliations

¹ 12nd Obstetrics & Gynaecology Department, Molecular & Morphological Clinical Cytopathology Laboratory, Hippokration General Hospital, Medical Faculty, School of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece.
² 21st Department of General Surgery, Anal & Colorectal Clinic, Papageorgiou General Hospital, Medical Faculty, School of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece.
³ 3Stomatology Clinic, School of Dentistry, Aristotle University of Thessaloniki, Thessaloniki, Greece.

Abstract

Background: Infection with human papillomaviruses (HPVs) can cause benign and malignant tumours in the anogenital tract and the oropharynx both in men and women. The aim of the presented study was to investigate cervical, anal, and oral HPV-detection rates among women referred to colposcopy for abnormal Cervical Cancer (CaCx) screening results and assess the concordance of HPV-types among these anatomical sites.

Methods: Women referred to colposcopy at a single centre due to abnormal cytology, conducted for CaCx screening, were subjected to cervical Liquid-based Cytology (LBC) smear testing, anal and oral sampling. Routine colposcopy consisted in multiple biopsies and/or Endocervical Curettage (ECC). HPV-detection was performed by PCR genotyping in all three anatomical sites. In high-risk (hr) HPV-DNA positive samples either from anal canal or oral cavity, anal LBC cytology and anoscopy were performed, or oral cavity examination respectively. Descriptive statistics was used for the analysis of HPV-detection rates and phi-coefficient for the determination of HPV-positivity concordance between the anatomical sites.

Results: Out of 118 referred women, hr. HPV-DNA was detected in 65 (55.1%), 64 (54.2%) and 3 (2.5%) at cervix, anal canal and oral cavity respectively while low-risk HPV-DNA was detected in 14 (11.9%) and 11 (9.3%) at cervix and anal canal respectively. The phi-coefficient for cervix/anal canal was 0.392 for HPV16, 0.658 for HPV31, 0.758 for HPV33, - 0.12 for HPV45, 0.415 for HPV52 and 0.473 for HPV58. All values were statistically significant (p < 0.001).

Conclusions: The results suggest that most HPV-types, high-risk and low-risk, detected in the cervix of women with prevalent cervical dysplasia, correlate with the ones detected in their anal canal. This particularly applies for the HPV-types included in the nonavalent HPV-vaccine (HPVs 6/11/16/18/31/33/45/52/58).

Keywords: Anal HPV-detection; Anal, cervical, oral HPV concordance; Cervical HPV-detection; Human papilloma virus; Oral HPV-detection.