Diagnosis of pulmonary tuberculosis from gastric aspirate samples in nonexpectorating pediatric patients in a tertiary care hospital

Indian J Pathol Microbiol. 2020 Apr-Jun;63(2):210-213. doi: 10.4103/IJPM.IJPM_694_19.

Abstract

Objectives: The aim of this study was to assess the utility of Xpert assay, Ziehl-Neelsen (ZN) staining, and Mycobacteria Growth Indicator Tube (MGIT™) culture for diagnosis of pediatric pulmonary tuberculosis from gastric aspirate (GA) samples and to compare Xpert assay and ZN staining with MGIT rapid liquid culture.

Materials and methods: GA samples from 210 nonexpectorating children, aged between 6 months to 12 years, presenting to the pediatric out-patient department (OPD) with clinical suspicion of tuberculosis (TB) were collected. The samples were tested by GeneXpert, ZN staining, and MGIT liquid culture.

Results: GeneXpert is a more sensitive method for rapid and early diagnosis of pediatric TB when compared with microscopy.

Keywords: GeneXpert; MGIT; ZN staining; pediatric TB.

MeSH terms

  • Child
  • Child, Preschool
  • Colony Count, Microbial
  • Female
  • Gastrointestinal Contents / microbiology*
  • Humans
  • Infant
  • Male
  • Mycobacterium tuberculosis / isolation & purification*
  • Nucleic Acid Amplification Techniques
  • Sensitivity and Specificity
  • Sputum / microbiology
  • Tertiary Care Centers
  • Tuberculosis, Pulmonary / diagnosis*