Generation of two control iPSC clones NCCSi008-A and NCCSi008-B from an individual of Indian ethnicity

Arun Vaidyanath; Mohsina Khan; Anjali Shiras

doi:10.1016/j.scr.2020.101765

Generation of two control iPSC clones NCCSi008-A and NCCSi008-B from an individual of Indian ethnicity

Stem Cell Res. 2020 May:45:101765. doi: 10.1016/j.scr.2020.101765. Epub 2020 Apr 14.

Authors

Arun Vaidyanath¹, Mohsina Khan¹, Anjali Shiras¹

Affiliation

¹ National Centre for Cell Science (NCCS), Ganeshkhind Road, Pune 411007 India.

PMID: 32315960
DOI: 10.1016/j.scr.2020.101765

Abstract

Two iPSC clones, NCCSi008-A and NCCSi008-B, were generated from a healthy male individual of Indian origin by reprogramming his CD4⁺ T cells with an integration free Sendai viral vector. The established iPSC clones showed high alkaline phosphatase (ALP) activity, expression of pluripotency markers, a normal male karyotype consistent with the donor gender (46, XY) and has potential for multi-lineage differentiation. These iPSC lines of Indian origin would serve as valuable resources for disease modeling, drug development and screening.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Cells, Cultured
Clone Cells
Ethnicity
Humans
Induced Pluripotent Stem Cells*
Male
Sendai virus