Genomic characterisation of a newly identified badnavirus infecting ivy (Hedera helix)

Arch Virol. 2020 Jun;165(6):1511-1514. doi: 10.1007/s00705-020-04627-1. Epub 2020 Apr 20.

Abstract

High-throughput sequencing (HTS) was used to investigate ringspots on ivy (Hedera helix) leaves. De novo assembly of HTS data generated from a total RNA extract from these leaves yielded a contig with sequence similarity to viruses of the genus Badnavirus, family Caulimoviridae. The complete genome sequence of this virus consists of 8,885 nucleotides and has three open reading frames (ORFs). Genome organisation and phylogenetic analysis identifies this newly identified virus as a new member of the genus Badnavirus for which we propose the name "ivy ringspot-associated virus" (IRSaV).

MeSH terms

  • Badnavirus / classification
  • Badnavirus / genetics*
  • Badnavirus / isolation & purification
  • Genome, Viral*
  • Hedera / virology*
  • High-Throughput Nucleotide Sequencing
  • Open Reading Frames
  • Phylogeny
  • Plant Diseases / virology*
  • Whole Genome Sequencing