Thiol-Poly(Sodium Styrene Sulfonate) (PolyNaSS-SH) Gold Complexes: From a Chemical Design to a One-Step Synthesis of Hybrid Gold Nanoparticles and Their Interaction with Human Proteins

Céline Falentin-Daudré; Mounia Aitouakli; Jean Sébastien Baumann; Nadia Bouchemal; Vincent Humblot; Véronique Migonney; Jolanda Spadavecchia

doi:10.1021/acsomega.0c00376

Thiol-Poly(Sodium Styrene Sulfonate) (PolyNaSS-SH) Gold Complexes: From a Chemical Design to a One-Step Synthesis of Hybrid Gold Nanoparticles and Their Interaction with Human Proteins

ACS Omega. 2020 Apr 2;5(14):8137-8145. doi: 10.1021/acsomega.0c00376. eCollection 2020 Apr 14.

Authors

Céline Falentin-Daudré¹, Mounia Aitouakli¹, Jean Sébastien Baumann¹, Nadia Bouchemal¹, Vincent Humblot², Véronique Migonney¹, Jolanda Spadavecchia¹

Affiliations

¹ CNRS, UMR 7244, NBD-LBPS-CSPBAT, Laboratoire de Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques Université Paris 13, Sorbonne Paris Cité, 93000 Bobigny, France.
² FEMTO-ST Institute, UMR CNRS 6174, Université Bourgogne Franche-Comté, 15B avenue des Montboucons, 25030 Besançon Cedex, France.

Abstract

This study highlights recent advances in the synthesis of nanoconjugates based on gold (Au(III)) complex with a bioactive polymer bearing sulfonate groups called thiol-poly(sodium styrene sulfonate) (PolyNaSS-SH) with various molecular weights (5, 10, and 35 kDa). The three nanomaterials differ substantially in shape and structure. In particular, for PolyNaSS-SH of 35 kDa, we obtained a characteristic core-shell flower shape after chelation of the Au(III) ions and successively reduction with sodium borohydride (NaBH₄). The mechanism of formation of the hybrid nanoparticles (PolyNaSS-SH@AuNPs (35 kDa) and their interactions between plasmatic proteins (human serum albumin (HSA), collagen I (Col 1), and fibronectin (Fn)) were deeply studied from a chemical and physical point of view by using several analytical techniques such as Raman spectroscopy, UV-visible, transmission electron microscopy (TEM), ¹H NMR, and X-ray photoelectron spectroscopy (XPS).