Abstract
Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) is rapidly spreading around the world. There is no existing vaccine or proven drug to prevent infections and stop virus proliferation. Although this virus is similar to human and animal SARS-CoVs and Middle East Respiratory Syndrome coronavirus (MERS-CoVs), the detailed information about SARS-CoV-2 proteins structures and functions is urgently needed to rapidly develop effective vaccines, antibodies, and antivirals. We applied high-throughput protein production and structure determination pipeline at the Center for Structural Genomics of Infectious Diseases to produce SARS-CoV-2 proteins and structures. Here we report two high-resolution crystal structures of endoribonuclease Nsp15/NendoU. We compare these structures with previously reported homologs from SARS and MERS coronaviruses.
Keywords:
COVID-19; EndoU family; NendoU; Nsp15; SARS-CoV-2; crystal structure; endoribonuclease.
© 2020 The Authors. Protein Science published by Wiley Periodicals, Inc. on behalf of The Protein Society.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Betacoronavirus / chemistry*
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Betacoronavirus / genetics
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Betacoronavirus / metabolism
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Catalytic Domain
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Cloning, Molecular
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Crystallography, X-Ray
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Endoribonucleases / chemistry*
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Endoribonucleases / genetics
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Endoribonucleases / metabolism
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Gene Expression
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Genetic Vectors / chemistry
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Genetic Vectors / metabolism
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Humans
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Middle East Respiratory Syndrome Coronavirus / chemistry*
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Middle East Respiratory Syndrome Coronavirus / genetics
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Middle East Respiratory Syndrome Coronavirus / metabolism
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Models, Molecular
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Oligonucleotides / chemistry*
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Oligonucleotides / metabolism
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Protein Binding
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Protein Conformation, alpha-Helical
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Protein Conformation, beta-Strand
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Protein Interaction Domains and Motifs
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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SARS-CoV-2
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Sequence Alignment
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Sequence Homology, Amino Acid
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Severe acute respiratory syndrome-related coronavirus / chemistry*
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Severe acute respiratory syndrome-related coronavirus / genetics
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Severe acute respiratory syndrome-related coronavirus / metabolism
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Substrate Specificity
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Viral Nonstructural Proteins / chemistry*
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Viral Nonstructural Proteins / genetics
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Viral Nonstructural Proteins / metabolism
Substances
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Oligonucleotides
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Recombinant Proteins
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Viral Nonstructural Proteins
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Endoribonucleases
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nidoviral uridylate-specific endoribonuclease