HSF1 is required for induction of mitochondrial chaperones during the mitochondrial unfolded protein response

Abstract

The mitochondrial unfolded protein response (UPR^mt ) is characterized by the transcriptional induction of mitochondrial chaperone and protease genes in response to impaired mitochondrial proteostasis and is regulated by ATF5 and CHOP in mammalian cells. However, the detailed mechanisms underlying the UPR^mt are currently unclear. Here, we show that HSF1 is required for activation of mitochondrial chaperone genes, including HSP60, HSP10, and mtHSP70, in mouse embryonic fibroblasts during inhibition of matrix chaperone TRAP1, protease Lon, or electron transfer complex 1 activity. HSF1 bound constitutively to mitochondrial chaperone gene promoters, and we observed that its occupancy was remarkably enhanced at different levels during the UPR^mt . Furthermore, HSF1 supported the maintenance of mitochondrial function under the same conditions. These results demonstrate that HSF1 is required for induction of mitochondrial chaperones during the UPR^mt , and thus, it may be one of the guardians of mitochondrial function under conditions of impaired mitochondrial proteostasis.

Keywords: HSF1; SSBP1; heat shock protein; mitochondria; proteostasis; proteotoxic stress.