Complete Genome Sequence of Staphylococcus aureus Phage SA75, Isolated from Goat Feces

Roshan D'Souza; Richard C White; Rachel Buzzeo; Karrie Goglin; Sanjay Vashee; Yoona Lee; Bokyung Son; Sangryeol Ryu; Derrick E Fouts

doi:10.1128/MRA.00114-20

Complete Genome Sequence of Staphylococcus aureus Phage SA75, Isolated from Goat Feces

Microbiol Resour Announc. 2020 Apr 16;9(16):e00114-20. doi: 10.1128/MRA.00114-20.

Authors

Roshan D'Souza¹, Richard C White¹, Rachel Buzzeo¹, Karrie Goglin², Sanjay Vashee¹, Yoona Lee³, Bokyung Son³, Sangryeol Ryu³, Derrick E Fouts⁴

Affiliations

¹ J. Craig Venter Institute, Rockville, Maryland, USA.
² J. Craig Venter Institute, La Jolla, California, USA.
³ Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea.
⁴ J. Craig Venter Institute, Rockville, Maryland, USA dfouts@jcvi.org.

Abstract

Antibiotic-resistant Staphylococcus aureus is an opportunistic pathogen causing serious human infections worldwide. Here, we report the complete annotated genome of bacteriophage SA75, a member of the Siphoviridae family which could be an alternative to traditional antibiotics for treating Staphylococcus infections. We used a hybrid approach combining MinION and Illumina MiSeq sequencing, which yielded a 43,134-bp genome and 65 open reading frames.