Microbiome in Intestinal Lavage Fluid May Be A Better Indicator in Evaluating The Risk of Developing Colorectal Cancer Compared with Fecal Samples

Weitao Shen; Jiayu Sun; Fen Yao; Kaihuang Lin; Yumeng Yuan; Yexi Chen; Hui Han; Zhiyang Li; Juan Zou; Xiaoyang Jiao

doi:10.1016/j.tranon.2020.100772

Microbiome in Intestinal Lavage Fluid May Be A Better Indicator in Evaluating The Risk of Developing Colorectal Cancer Compared with Fecal Samples

Transl Oncol. 2020 May;13(5):100772. doi: 10.1016/j.tranon.2020.100772. Epub 2020 Apr 13.

Authors

Weitao Shen¹, Jiayu Sun², Fen Yao³, Kaihuang Lin⁴, Yumeng Yuan⁵, Yexi Chen⁶, Hui Han⁷, Zhiyang Li⁸, Juan Zou⁹, Xiaoyang Jiao¹⁰

Affiliations

¹ The second affiliated hospital of Shantou University Medical College, Shantou, Guangdong, China 515041. Electronic address: 646957018@qq.com.
² Department of Cell Biology and Genetics, Shantou University Medical College, Shantou, Guangdong, China 515041. Electronic address: 18jysun1@stu.edu.cn.
³ Department of Cell Biology and Genetics, Shantou University Medical College, Shantou, Guangdong, China 515041. Electronic address: fyao@stu.edu.cn.
⁴ The second affiliated hospital of Shantou University Medical College, Shantou, Guangdong, China 515041. Electronic address: linkaihuang@163.com.
⁵ Department of Cell Biology and Genetics, Shantou University Medical College, Shantou, Guangdong, China 515041. Electronic address: 671421696@qq.com.
⁶ The second affiliated hospital of Shantou University Medical College, Shantou, Guangdong, China 515041. Electronic address: chenyexi@vip.sina.com.
⁷ The second affiliated hospital of Shantou University Medical College, Shantou, Guangdong, China 515041. Electronic address: hanhui0571@126.com.
⁸ The second affiliated hospital of Shantou University Medical College, Shantou, Guangdong, China 515041. Electronic address: medyzy@163.com.
⁹ The second affiliated hospital of Shantou University Medical College, Shantou, Guangdong, China 515041. Electronic address: 15271689450@163.com.
¹⁰ Department of Cell Biology and Genetics, Shantou University Medical College, Shantou, Guangdong, China 515041. Electronic address: xyjiao@stu.edu.cn.

Abstract

Objective: Intestinal microbiota plays a vital role in the pathogenesis of colorectal cancer (CRC), which is crucial for assessing the risk and prognosis of CRC. Most studies regarding human gut microbiota mainly based on the feces, but the exact composition of microbiota vary significantly due to fecal composition is easily affected by many factors. We aim to evaluate whether intestinal lavage fluid (IVF) is a better substitution mirroring the gut microbiota.

Methods: We performed 16S rRNA gene analysis on fecal and IVF samples from 30 CRC patients and 25 healthy individuals, comparison in luminal (feces) / mucosal (IVF) adherent bacterial community profiles were analyzed.

Results: The difference between feces and IVF were observed, including the diversity and abundance of pathogenic bacteria (either in single strain or in co-occurrence pattern). IVF group shared 605 OTUs with the fecal group, but there was 94 OTUs only observed in fecal samples, while 247 OTUs were mainly existing in the IVF group. Among them, 27 vital bacterial species detected in IVF, while 10 critical species detected in fecal samples. The co-occurrence bacteria Fusobacteria Cluster and Proteobacteria Cluster 2 significantly increased in IVF than in control (P < .01), while Firmicutes Cluster 1, Firmicutes Cluster 2 and Proteobacteria Cluster 1 were markedly lower in IVF than in control (P < .001). In CRC feces, Fusobacteria Cluster was higher than in control (P < .05), but Firmicutes Cluster 1 was of substantially less abundance than in control (P < .001). Proteobacteria Cluster 2 was increased dramatically in IVF than in feces (P < .05), Firmicutes Cluster 1 were of substantially less abundance than in feces (P < .05).

Conclusion: Pathogenic microbiota is more abundant in IVF than in feces. Microbiota of IVF may closely be related to the mucosal-associated microbial communities, which benefit from elucidating the relationship of the intestinal microbiota and CRC carcinogenesis.