Response of an aerobic granular and conventional flocculated reactors against changing feed composition from simple composition to more complex

Abstract

This research evaluated the effect of changing feed composition on the performances of a conventional activated sludge (CAS) and an aerobic granular sludge (AGS) reactor operated simultaneously. Both reactors were initially fed with 100% synthetic feed. In a stepwise manner, the feed composition was slowly changed to real primary effluent collected from a local wastewater treatment plant. After an initial stabilization period, both reactors could achieve more than 90% NH₄⁺-N removal. However, PO₄^3--P removal eventually reached to a maximum of 92% in the AGS and 88% in the CAS. COD removal in both reactors was least affected, with the lowest percent removal of 81 ± 3% achieved in AGS and 62 ± 4% in CAS respectively when fed with 100% real wastewater. Despite granule breakage the AGS reactor was able to remove the pollutants (COD, N, P). The abundance of Candidatus Accumulibacter, a polyphosphate accumulating organism, in the AGS system increased over the operational phases: II (6.2%), III (10.32%), and IV (11.9%). While in CAS, it increased from phase I to phase II (12.6%), but decreased in phase III to 9.9%. Genus-based classification revealed a successive increase in the relative abundance of Nitrospira to 11.05% during Phase III and 10.3% during Phase IV in the AGS. In contrast with its presence in the CAS, which was, 3.4% during Phase III and 9.5% during Phase IV.

Keywords: Candidatus accumulibacter phosphatis; Conventional activated sludge; Denitrifying PAOs; Granular sludge; Microbial ecology.