Abstract
A photoactivatable fluorogenic tetrazine-rhodaphenothiazine probe was synthesized and studied in light-assisted, bioorthogonal labeling schemes. Experimental results revealed that the bioorthogonally conjugated probe efficiently sensitizes 1O2 generation upon illumination with green or orange light and undergoes self-oxidation leading to an intensely fluorescent sulfoxide product. An added value of the present probe is that it is also suitable for STED super-resolution microscopy using a 660 nm depletion laser.
MeSH terms
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Animals
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COS Cells
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Chlorocebus aethiops
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Fluorescent Dyes / chemistry*
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Fluorescent Dyes / radiation effects
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Lasers
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Light
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Microscopy, Confocal / instrumentation
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Microscopy, Confocal / methods
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Oxidation-Reduction / radiation effects
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Phenothiazines / chemistry*
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Phenothiazines / radiation effects
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Photosensitizing Agents / chemistry*
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Photosensitizing Agents / radiation effects
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Rhodamines / chemistry*
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Rhodamines / radiation effects
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Singlet Oxygen / chemistry
Substances
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Fluorescent Dyes
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Phenothiazines
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Photosensitizing Agents
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Rhodamines
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Singlet Oxygen