Molecular Characteristics of Brucella Isolates Collected From Humans in Hainan Province, China

Zhenjun Li; Xu-Ming Wang; Xiong Zhu; Miao Wang; Hai Cheng; Dan Li; Zhi Guo Liu

doi:10.3389/fmicb.2020.00452

Molecular Characteristics of Brucella Isolates Collected From Humans in Hainan Province, China

Front Microbiol. 2020 Mar 27:11:452. doi: 10.3389/fmicb.2020.00452. eCollection 2020.

Authors

Zhenjun Li¹, Xu-Ming Wang², Xiong Zhu³, Miao Wang⁴, Hai Cheng³, Dan Li¹, Zhi Guo Liu^{1

5}

Affiliations

¹ State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
² Hainan Provincial People's Hospital, Haikou, China.
³ Sanya People's Hospital, Sanya, China.
⁴ Ulanqab Centre for Endemic Disease Prevention and Control, Jining, China.
⁵ Inner Mongolia Autonomous Region Center for Comprehensive Disease Control and Prevention, Huhhot, China.

Abstract

Brucellosis has been reported in several regions of Hainan Province, but the extent of the disease has not been fully elucidated. Conventional biotyping methods, multiple locus variable number tandem repeats analysis (MLVA), and single-nucleotide polymorphisms (SNPs) from draft genome sequencing were employed to characterize the strains. There were four biovars (Brucella melitensis bv. 1, 2, and 3 and Brucella suis bv. 3) detected, which showed that the biovar diversity of Brucella in Hainan is higher than in other areas of China. Both B. melitensis bv. 3 and B. suis bv. 3 were dominant species and showed epidemiology patterns that were compatible with both southern and northern China. Eight of MLVA-11 genotypes were known (31, 111, 116, 120, 136, 291, 297, and 345), and the remaining seven were novel (HN11-1 to HN11-7); these data showed that Brucella strains in this study had multiple geographic origins and exhibited characteristics of origin and evolution of co-existing imported and Hainan specific lineage. A total of 41 strains were found, belonging to 37 unique genotypes that each represented a single strain, which suggests that these strains were not directly related epidemiologically and indicates that the epidemic characteristics of human brucellosis in Hainan was dominated by sporadic strains. The high HGDI values were observed in MLVA-8, MLVA-11, and MLVA-16 among two species, suggesting considerable genetic diversity among these species. MST is characterized based on MLVA-16 that was found both throughout China and on a global level and showed that strains of this study had significant genetic differences with strains from many parts of the globe and seemingly represent a unique genetic lineage. Whole-genome SNP analysis showed that four B. melitensis were closely related to strains from China's northern provinces, and the source of infection was partly of human brucellosis in this province that may have been from these regions. The B. suis were closely related to strains from the United States, and further investigation of the transportation of animals, such as pigs, is needed to elucidate the origins of these strains.

Keywords: Brucella melitensis; Brucella suis; Hainan; multiple loci variable number tandem repeats analysis; single-nucleotide polymorphism.