Rice OsBT1 regulates seed dormancy through the glycometabolism pathway

Weihan Song; Qixian Hao; Mengying Cai; Yihua Wang; Xingjie Zhu; Xi Liu; Yunshuai Huang; Thanhliem Nguyen; Chunyan Yang; Jiangfeng Yu; Hongming Wu; Liangming Chen; Yunlu Tian; Ling Jiang; Jianmin Wan

doi:10.1016/j.plaphy.2020.03.055

Rice OsBT1 regulates seed dormancy through the glycometabolism pathway

Plant Physiol Biochem. 2020 Jun:151:469-476. doi: 10.1016/j.plaphy.2020.03.055. Epub 2020 Apr 5.

Authors

Affiliations

¹ State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing, 210095, China.
² State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing, 210095, China; Department of Biology and Agricultural Engineering, Quynhon University, Quynhon, Binhdinh, 590000, Viet Nam.
³ State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: jiangling@njau.edu.cn.
⁴ State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing, 210095, China; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. Electronic address: wanjm@njau.edu.cn.

PMID: 32289640
DOI: 10.1016/j.plaphy.2020.03.055

Abstract

Seed dormancy and germination in rice (Oryza sativa L.) are complex and important agronomic traits that involve a number of physiological processes and energy. A mutant named h470 selected from a⁶⁰Co-radiated indica cultivar N22 population had weakened dormancy that was insensitive to Gibberellin (GA) and Abscisic acid (ABA). The levels of GA₄ and ABA were higher in h470 than in wild-type (WT) plants. The gene controlling seed dormancy in h470 was cloned by mut-map and transgenesis and confirmed to encode an ADP-glucose transporter protein. A 1 bp deletion in Os02g0202400 (OsBT1) caused the weaker seed dormancy in h470. Metabolomics analyses showed that most sugar components were higher in h470 seeds than the wild type. The mutation in h470 affected glycometabolism.

Keywords: Germination; Glycometabolism; Oryza sativa; OsBT1.

MeSH terms

Abscisic Acid / pharmacology
Gene Expression Regulation, Plant
Germination
Gibberellins / pharmacology
Oryza* / physiology
Plant Dormancy* / drug effects
Plant Dormancy* / genetics
Plant Growth Regulators / pharmacology
Plant Proteins* / genetics
Seeds* / genetics

Substances

Gibberellins
Plant Growth Regulators
Plant Proteins
Abscisic Acid