Effects of Curcumin and Pentagamavunon-0 Against Dengue-2 Virus Infection in Vero Cells; an in Vitro Study

Dewi Marbawati; Sitti Rahmah Umniyati

doi:10.1016/j.proenv.2015.01.033

Effects of Curcumin and Pentagamavunon-0 Against Dengue-2 Virus Infection in Vero Cells; an in Vitro Study

Procedia Environ Sci. 2015:23:215-221. doi: 10.1016/j.proenv.2015.01.033. Epub 2015 Jan 31.

Authors

Dewi Marbawati¹, Sitti Rahmah Umniyati²

Affiliations

¹ Vector Borne Disease Control Research and Development Office in Banjarnegara, Selamanik Street Banjarnegara 53415, Indonesia.
² Departemen of Parasitology, Faculty of Medicine, Universitas Gadjah Mada, Sekip Utara, Yogyakarta,55281, Indonesia.

Abstract

The research aims was to determine the cytotoxic effects and the highest safe concentration of curcumin and PGV-0 on vero cells. This research also compare the effect of curcumin and PGV-0 against vero cells infected by Dengue virus (in vitro) in one and three days incubation period. The highest safe concentrations against vero cells from curcumin was 6.25 ppm and 1.5625 ppm for PGV-0. Immunocytochemistry test of curcumin and PGV-0 in both incubation period (one and three days) shows no significant difference (significant value 0.925), but RT-PCR result indicates that PGV - 0 have potential antiviral better than curcumin.

Keywords: Dengue; PGV-0; RT-PCR; curcumin; immunocytochemistry.