A novel application of small-angle scattering techniques: Quality assurance testing of virus quantification technology

Deborah A Kuzmanovic; Ilya Elashvili; Catherine O'Connell; Susan Krueger

doi:10.1016/j.radphyschem.2007.10.004

A novel application of small-angle scattering techniques: Quality assurance testing of virus quantification technology

Radiat Phys Chem Oxf Engl 1993. 2008 Mar;77(3):215-224. doi: 10.1016/j.radphyschem.2007.10.004. Epub 2007 Oct 23.

Authors

Deborah A Kuzmanovic¹, Ilya Elashvili², Catherine O'Connell¹, Susan Krueger³

Affiliations

¹ Biotechnology Division, NIST, 100 Bureau Drive, Stop 8311, Gaithersburg, MD 20899-8311, USA.
² Edgewood Chemical Biological Center; 5183 Blackhawk Road, Aberdeen Proving Ground, MD 21010, USA.
³ NIST Center for Neutron Research, NIST, 100 Bureau Drive, Stop 6102, Gaithersburg, MD 20899-6102, USA.

Abstract

Small-angle scattering (SAS) techniques, like small-angle X-ray scattering (SAXS) and small-angle neutron scattering (SANS), were used to measure and thus to validate the accuracy of a novel technology for virus sizing and concentration determination. These studies demonstrate the utility of SAS techniques for use in quality assurance measurements and as novel technology for the physical characterization of viruses.

Keywords: Bacteriophage MS2; Charged reduced electrospray size spectrometry; Gas-phase electrophoretic mobility macromolecular analysis; Small-angle scattering; Virus concentration.