Virus purification and enrichment by hydroxyapatite chromatography on a chip

Miyako Niimi; Taisuke Masuda; Kunihiro Kaihatsu; Nobuo Kato; Shota Nakamura; Takaaki Nakaya; Fumihito Arai

doi:10.1016/j.snb.2014.04.011

Virus purification and enrichment by hydroxyapatite chromatography on a chip

Sens Actuators B Chem. 2014 Oct 1:201:185-190. doi: 10.1016/j.snb.2014.04.011. Epub 2014 May 6.

Authors

Miyako Niimi¹, Taisuke Masuda², Kunihiro Kaihatsu³, Nobuo Kato³, Shota Nakamura⁴, Takaaki Nakaya⁵, Fumihito Arai²

Affiliations

¹ Department of Mechanical Science and Engineering, Graduate School of Engineering, Nagoya University, 1 Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
² Department of Micro-Nano Systems Engineering, Graduate School of Engineering, Nagoya University, 1 Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
³ The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.
⁴ Department of Infection Metagenomics, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
⁵ Department of Infectious Diseases, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan.

Abstract

The spread of infectious diseases has become a global health concern. In order to diagnose infectious diseases quickly and accurately, next-generation DNA sequencing techniques for genetic analysis of infectious viruses have been developed rapidly. However, it takes a very long time to pretreat clinical samples for genetic analysis using next-generation sequencers. We have therefore developed a microfluidic chromatography chip that can purify and enrich viruses in a sample using hydroxyapatite particles packed in a micro-column. We demonstrated the purification of virus from a mixture of virus and FBS protein, and enrichment of the virus using this novel microfluidic chip.

Keywords: Hydroxyapatite chromatography; Infectious disease; Microfluidic chip; Virus enrichment; Virus purification.