Plant tissue distribution and chemical inactivation of six carnation viruses

Jesús A Sánchez-Navarro; M Carmen Cañizares; Emilio A Cano; Vicente Pallás

doi:10.1016/j.cropro.2006.09.016

Plant tissue distribution and chemical inactivation of six carnation viruses

Crop Prot. 2007 Jul;26(7):1049-1054. doi: 10.1016/j.cropro.2006.09.016. Epub 2006 Dec 11.

Authors

Jesús A Sánchez-Navarro¹, M Carmen Cañizares², Emilio A Cano³, Vicente Pallás¹

Affiliations

¹ Instituto de Biología Molecular y Celular de Plantas IBMCP (UPV-CSIC), Av de los Naranjos s/n 46022 Valencia, Spain.
² Estación Experimental la Mayora (CSIC), Algarrobo-Costa 29750 (Málaga), Spain.
³ Departamento de I+D, Barberet and Blanc S.A., Apartado de Correos 38, 30890 Puerto Lumbreras (Murcia), Spain.

Abstract

Carnation mottle virus (CarMV), Carnation etched ring virus (CERV), Carnation vein mottle virus (CVMV), Carnation ringspot virus (CRSV), Carnation Italian ringspot virus (CIRV) and Carnation latent virus (CLV) are the most important viruses affecting carnation crops. All except CERV are RNA viruses. Viral RNA or DNA accumulation on root, stem, leaf, sepal, petal, stamen, pistil and ovary tissues of infected carnation or Saponaria vaccaria plants was analysed by non-isotopic molecular hybridisation. High-titres of CarMV, CRSV, CIRV, and CLV accumulated in all plant tissues whereas CERV and CVMV were irregularly distributed over the plant. High-titres of all viruses accumulated in leaf, petal, stamen, pistil, and ovary tissues, so leaves or petals are a good tissue for routine diagnosis. Six chemicals were evaluated for inactivation of all carnation viruses in infected extracts. Commercial bleach at 7% (v/v) or NaOH at 0.5% (w/v) was found to inactivate all viruses after 60 s treatment in a systemic S. vaccaria bioassay.

Keywords: Carnation viruses; Chemical inactivation; Virus distribution.