Intravascular ultrasound (IVUS) tissue harmonic imaging (THI) is a useful vessel imaging technique that can provide deep penetration depth as well as high spatial and contrast resolution. Typically, a high-frequency IVUS transducer for THI requires a broad bandwidth or dual-frequency bandwidth. However, it is very difficult to make an IVUS transducer with a frequency bandwidth covering from the fundamental frequency to the second harmonic or a dual-peak at the desired frequency. To solve this problem, in this study, we applied the polarization inversion technique (PIT) to the IVUS transducer for THI. The PIT makes it relatively easy to design IVUS transducers with suitable frequency characteristics for THI depending on the inversion ratio of the piezoelectric layer and specifications of the passive materials. In this study, two types of IVUS transducers based on the PIT were developed for THI. One is a front-side inversion layer (FSIL) transducer with a broad bandwidth, and the other is a back-side inversion layer (BSIL) transducer with a dual-frequency bandwidth. These transducers were designed using finite element analysis (FEA)-based simulation, and the prototype transducers were fabricated. Subsequently, the performance was evaluated by not only electrical impedance and pulse-echo response tests but also B-mode imaging tests with a 25 μm tungsten wire and tissue-mimicking gelatin phantoms. The FEA simulation and experimental results show that the proposed scheme can successfully implement the tissue harmonic IVUS image, and thus it can be one of the promising techniques for developing IVUS transducers for THI.