Application of Fusarium sp. immobilized on multi-walled carbon nanotubes for solid-phase extraction and trace analysis of heavy metal cations

Hossein Moallaei; Jean-Philippe Bouchara; Abolfazl Rad; Pardeep Singh; Pankaj Raizada; Hai Nguyen Tran; Muhammad Nadeem Zafar; Dimitrios A Giannakoudakis; Ahmad Hosseini-Bandegharaei

doi:10.1016/j.foodchem.2020.126757

Application of Fusarium sp. immobilized on multi-walled carbon nanotubes for solid-phase extraction and trace analysis of heavy metal cations

Food Chem. 2020 Aug 30:322:126757. doi: 10.1016/j.foodchem.2020.126757. Epub 2020 Apr 6.

Authors

Hossein Moallaei¹, Jean-Philippe Bouchara², Abolfazl Rad³, Pardeep Singh⁴, Pankaj Raizada⁴, Hai Nguyen Tran⁵, Muhammad Nadeem Zafar⁶, Dimitrios A Giannakoudakis⁷, Ahmad Hosseini-Bandegharaei⁸

Affiliations

¹ Department of Microbiology, Immunology and Parasitology, Faculty of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran; Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran.
² Host-Pathogen Interaction Study Group (GEIHP, EA 3142), SFR ICAT 4208, Univ Angers, Univ Brest, Angers University Hospital, Angers, France.
³ Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran.
⁴ School of Chemistry, Faculty of Basic Sciences, Shoolini University, Solan, HP, India.
⁵ Institute of Fundamental and Applied Sciences, Duy Tan University, Ho Chi Minh City, 70000, Vietnam; Faculty of Environment and Chemical Engineering, Duy Tan University, Da Nang, 550000, Vietnam.
⁶ Department of Chemistry, University of Gujrat, 50700 Gujrat, Pakistan.
⁷ Laboratory of General & Inorganic Chemical Technology, Department of Chemistry, Aristotle University of Thessaloniki, GR 54124, Greece.
⁸ Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran; Department of Engineering, Kashmar Branch, Islamic Azad University, PO Box 161, Kashmar, Iran. Electronic address: ahoseinib@yahoo.com.

PMID: 32283378
DOI: 10.1016/j.foodchem.2020.126757

Abstract

In the present work, for the first time, the filamentous fungus Fusarium sp. was utilized for devising a novel method for pre-concentration and determination of trace amounts of Pb(II), Cu(II), Cd(II), and Zn(II) ions, using a mini-column packed with Fusarium-coated multi-walled carbon nanotubes and inductively coupled plasma-optical emission spectrometry. Optimal analytical conditions including pH, ionic strength, elution solution, sample and eluent flow rates, and sample volume were determined. The detection limits were 0.39, 0.060, 0.021, and 0.025 ng mL^-1 for Pb(II), Cu(II), Cd(II), and Zn(II) cations, respectively. This new method demonstrated a high performance for the analytes, and their adsorption was not affected by the different co-existing ions. The present procedure was validated by the analysis of standard reference materials, since the obtained data were in close agreement with reference values. Finally, this new procedure was successfully applied to analysis of heavy metal cations in natural food and water samples.

Keywords: Fusarium; Heavy metals ions; Multi-walled carbon nanotubes; Pre-concentration.

MeSH terms

Adsorption
Cations / chemistry
Cells, Immobilized / chemistry
Copper / analysis
Fresh Water / analysis
Fusarium / chemistry*
Hydrogen-Ion Concentration
Lead / analysis
Lead / isolation & purification
Limit of Detection
Metals, Heavy / analysis*
Metals, Heavy / isolation & purification
Metals, Heavy / standards
Nanotubes, Carbon / chemistry*
Osmolar Concentration
Reference Standards
Solid Phase Extraction*
Spectrophotometry / methods*
Spectrophotometry / standards

Substances

Cations
Metals, Heavy
Nanotubes, Carbon
Lead
Copper