Low Intensity Ultrasound Induces Epithelial Cell Adhesion Responses

Jormay Lim; Yeh-Shiu Chu; Ya-Cherng Chu; Chun-Min Lo; Jaw-Lin Wang

doi:10.1115/1.4046883

Low Intensity Ultrasound Induces Epithelial Cell Adhesion Responses

J Biomech Eng. 2020 Sep 1;142(9):091014. doi: 10.1115/1.4046883.

Authors

Jormay Lim¹, Yeh-Shiu Chu², Ya-Cherng Chu¹, Chun-Min Lo³, Jaw-Lin Wang⁴

Affiliations

¹ Department of Biomedical Engineering, National Taiwan University, 605 Jen-Su Hall, 1 Section 4, Roosevelt Road, Taipei 10617, Taiwan.
² Brian Research Center, National Yang-Ming University, No. 155, Sec. 2, Linong Street, Taipei 112, Taiwan.
³ Department of Biomedical Engineering, National Yang-Ming University, No. 155, Sec. 2, Linong Street, Taipei 112, Taiwan.
⁴ Department of Biomedical Engineering, National Taiwan University, 602 Jen-Su Hall, 1 Section 4, Roosevelt Road, Taipei 10617, Taiwan.

PMID: 32280990
DOI: 10.1115/1.4046883

Abstract

In this study, we investigated the cellular mechanosensitive responses to a low intensity ultrasound (LIUS) stimulation (ISATA = 1 mW/cm2, pressure = 10 kPa). The dose and temporal effects at cell-substrate adhesion (CSA) at the basal level and cell-cell adhesion (CCA) at the apical level are reported in detail. A model of mouse mammary gland epithelial cells (EpH4) and the phosphorylation of mechanosensitive 130 kDa Crk-associated substrate (p130CAS) as an indicator for cellular responses were used. The intensity of phospho-p130CAS was found to be dependent on LIUS stress level, and the p130CAS was phosphorylated after 1 min stimulation at CSA. The phospho-p130CAS was also found to increase significantly at CCA upon LIUS stimulation. We confirmed that the cellular responses to ultrasound are immediate and dose dependent. Ultrasound affects not only CSA but also CCA. An E-cadherin knockout (EpH4ECad-/-) model also confirmed that phosphorylation of p130CAS at CCA is related to E-cadherins.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cadherins / metabolism
Cell Adhesion
Crk-Associated Substrate Protein*
Mice
Phosphorylation
Signal Transduction

Substances

Cadherins
Crk-Associated Substrate Protein