pHEMA@AGMNA-1: A novel material for the development of antibacterial contact lens

A K Rossos; C N Banti; A G Kalampounias; C Papachristodoulou; K Kordatos; P Zoumpoulakis; T Mavromoustakos; N Kourkoumelis; S K Hadjikakou

doi:10.1016/j.msec.2020.110770

pHEMA@AGMNA-1: A novel material for the development of antibacterial contact lens

Mater Sci Eng C Mater Biol Appl. 2020 Jun:111:110770. doi: 10.1016/j.msec.2020.110770. Epub 2020 Feb 24.

Authors

A K Rossos¹, C N Banti², A G Kalampounias³, C Papachristodoulou⁴, K Kordatos⁵, P Zoumpoulakis⁶, T Mavromoustakos⁷, N Kourkoumelis⁸, S K Hadjikakou⁹

Affiliations

¹ Section of Inorganic and Analytical Chemistry, Department of Chemistry, University of Ioannina, Greece.
² Section of Inorganic and Analytical Chemistry, Department of Chemistry, University of Ioannina, Greece. Electronic address: cbanti@uoi.gr.
³ Physical Chemistry Laboratory, Department of Chemistry, University of Ioannina, Greece.
⁴ Department of Physics, University of Ioannina, Greece.
⁵ Laboratory of Inorganic and Analytical Chemistry, School of Chemical Engineering, National Technical University of Athens, Greece.
⁶ Institute of Biology, Medicinal Chemistry and Biotechnology, National Hellenic Research Foundation, Athens, Greece.
⁷ Organic Chemistry Laboratory, Department of Chemistry, National and Kapodistrian University of Athens Greece, Greece.
⁸ Medical Physics Laboratory, Medical School, University of Ioannina, Greece.
⁹ Section of Inorganic and Analytical Chemistry, Department of Chemistry, University of Ioannina, Greece; University Research Center of Ioannina (URCI), Institute of Materials Science and Computing, Ioannina, Greece. Electronic address: shadjika@uoi.gr.

PMID: 32279741
DOI: 10.1016/j.msec.2020.110770

Abstract

The Metal Organic Framework (MOF) of formula {[Ag₆(μ₃-HMNA)₄(μ₃-MNA)₂]^2-·[(Et₃NH)⁺]₂·(DMSO)₂·(H₂O)} (AGMNA), a known efficient antimicrobial compound which contains the anti-metabolite, 2-thio-nicotinic acid (H₂MNA), was incorporated in polymer hydrogels using, hydroxyethyl-methacrylate (HEMA). The material pHEMA@AGMNA-1 was characterized by X-ray fluorescence (XRF) spectroscopy, X-ray powder diffraction analysis (XRPD), Scanning Electron Microscopy (SEM), Energy-dispersive X-ray spectroscopy (EDX), Thermogravimetric Differential Thermal Analysis (TG-DTA), Differential Scanning Calorimetry (DTG/DSC), attenuated total reflection spectroscopy (FT-IR-ATR) and Ultrasonic Imaging. The antimicrobial capacity of pHEMA@AGMNA-1 was evaluated against the Gram negative bacterial strain Pseudomonas aeruginosa and the Gram positive ones of the genus of Staphylococcus epidermidis and Staphylococcus aureus, which are the etiology of the microbial keratitis. The % bacterial viability of P. aeruginosa, S. epidermidis and S. aureus upon their incubation with pHEMA@AGMNA-1 discs is significantly low (0.4 ± 0.1%, 1.5 ± 0.4% and 7.7 ± 0.5% respectively). The inhibition zones (IZ) caused by pHEMA@AGMNA-1 discs against P. aeruginosa, S. epidermidis and S. aureus are 14.0 ± 1.1, 11.3 ± 1.3 and 11.8 ± 1.8 mm respectively. Furthermore, pHEMA@AGMNA-1 exhibits low toxicity. Thus, pHEMA@AGMNA-1 might be an efficient candidate for the development of antimicrobial active contact lenses.

Keywords: Antibacterial active contact lens; Antimicrobial materials; Hydrogels; Microbial keratitis.

MeSH terms

Animals
Anti-Bacterial Agents / chemistry*
Anti-Bacterial Agents / pharmacology
Artemia / drug effects
Artemia / growth & development
Calorimetry, Differential Scanning
Cell Survival / drug effects
Contact Lenses, Hydrophilic / microbiology*
Epithelial Cells / cytology
Epithelial Cells / drug effects
Epithelial Cells / metabolism
Gram-Negative Bacteria / drug effects
Gram-Positive Bacteria / drug effects
Humans
Hydrogels / chemistry
Larva / drug effects
Metal-Organic Frameworks / chemistry*
Polyhydroxyethyl Methacrylate / chemistry*
Spectroscopy, Fourier Transform Infrared

Substances

Anti-Bacterial Agents
Hydrogels
Metal-Organic Frameworks
Polyhydroxyethyl Methacrylate