Dynamic monitoring and quantitative characterization of intracellular H2O2 content by using SERS based boric acid nanoprobe

Wendai Cheng; Ping Tang; Xuanmeng He; Xinyue Xing; Meishuang Xing; Liupeng Lu; Peng Ju; Yi Wang; Shengde Liu; Xiaoxu Lu; Liyun Zhong

doi:10.1016/j.talanta.2020.120863

Dynamic monitoring and quantitative characterization of intracellular H₂O₂ content by using SERS based boric acid nanoprobe

Talanta. 2020 Jul 1:214:120863. doi: 10.1016/j.talanta.2020.120863. Epub 2020 Feb 26.

Authors

Wendai Cheng¹, Ping Tang¹, Xuanmeng He², Xinyue Xing¹, Meishuang Xing¹, Liupeng Lu¹, Peng Ju¹, Yi Wang¹, Shengde Liu¹, Xiaoxu Lu¹, Liyun Zhong³

Affiliations

¹ Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou, 510006, China.
² Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou, 510006, China; Brain Academy of South China Normal University, Guangzhou, 510631, China.
³ Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou, 510006, China. Electronic address: zhongly@scnu.edu.cn.

PMID: 32278437
DOI: 10.1016/j.talanta.2020.120863

Abstract

Quantitative characterization of intracellular H₂O₂ content, which is still difficult by the conventional biochemical methods due to the lack of real-time and non-invasive technique of single cell measurement, is a useful solution for cell state assessment. Based on the surface enhanced Raman scattering (SERS), we construct a novel boric acid (BA) nanoprobe to perform quantitative characterization of H₂O₂ content, in which the p-thiol benzene boric acid (4-MPBA) reporter molecule modified with gold nanorods (AuNRs) is employed for Raman signal enhancement. The achieved result demonstrates obvious advantages of the synthesized AuNRs/4-MPBA/BA nanoprobe in measurement sensitivity of H₂O₂ content. Importantly, this AuNRs/4-MPBA/BA nanoprobe will provide a powerful tool for dynamic monitoring and quantitative characterization of intracellular H₂O₂ content during cell apoptosis or other cell growth processes, and then achieve important reference data for studying the corresponding molecular mechanism.

Keywords: Boric acid; Cell apoptosis; Nanoprobe; Paclitaxel; Raman spectral imaging; Surface enhanced Raman scattering (SERS).

MeSH terms

Apoptosis
Boronic Acids / chemical synthesis
Boronic Acids / chemistry*
Cell Proliferation
Fluorescent Dyes / chemical synthesis
Fluorescent Dyes / chemistry*
Gold / chemistry
HeLa Cells
Humans
Hydrogen Peroxide / analysis*
Metal Nanoparticles / chemistry
Molecular Structure
Nanoparticles / chemistry*
Particle Size
Spectrum Analysis, Raman
Surface Properties
Tumor Cells, Cultured

Substances

Boronic Acids
Fluorescent Dyes
Gold
Hydrogen Peroxide