A float culture method for fungal secondary metabolism study using hydrophilic polyvinylidene fluoride membranes

Fei Tian; Sang Yoo Lee; So Young Woo; Hwa Young Choi; Hyang Sook Chun

doi:10.1016/j.ab.2020.113722

A float culture method for fungal secondary metabolism study using hydrophilic polyvinylidene fluoride membranes

Anal Biochem. 2020 Jun 15:599:113722. doi: 10.1016/j.ab.2020.113722. Epub 2020 Apr 8.

Authors

Fei Tian¹, Sang Yoo Lee¹, So Young Woo¹, Hwa Young Choi¹, Hyang Sook Chun²

Affiliations

¹ Advanced Food Safety Research Group, BK21 Plus, School of Food Science and Technology, Chung-Ang University, Anseong, 17546, South Korea.
² Advanced Food Safety Research Group, BK21 Plus, School of Food Science and Technology, Chung-Ang University, Anseong, 17546, South Korea. Electronic address: hschun@cau.ac.kr.

PMID: 32275893
DOI: 10.1016/j.ab.2020.113722

Abstract

Fungal metabolism is affected by both the developmental stage and cultivation conditions. Fungal growth in solid culture reflects natural conditions more closely than growth in liquid culture; however, because the mycelium cannot be harvested easily and the medium composition cannot be modified during incubation, the approach has some limitations when compared to liquid culture methods. The float culture incubation method introduced herein enables fungus to develop similar colonies to those on solid culture. This is a simple method that leads to the production of high-quality RNA samples.

Keywords: Aflatoxin; Colony morphology; Fungal incubation; Gene expression; Polyvinylidene fluoride membrane.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aflatoxins / biosynthesis*
Aspergillus flavus* / growth & development
Aspergillus flavus* / metabolism
Culture Media*
Microbiological Techniques / methods*
Secondary Metabolism

Substances

Aflatoxins
Culture Media