Application of anaerobic bacterial ammonification pretreatment to microalgal food waste leachate cultivation and biofuel production

Abstract

Food waste constitutes the largest component of municipal solid waste in many urbanized societies. The current practice of disposing of biodegradable food waste mixed with other solid wastes to landfills is not sustainable and is environmentally undesirable. Moreover, the leakage of nutrient-rich food waste leachate (FWL) impacts the environment by eutrophication of the water body. Two robust microalgal species, Dunaliella tertiolecta (D. tertiolecta) and Cyanobacterium aponinum (C. aponinum), have been selected previously for the treatment of FWL because they can tolerate diluted FWL. However, growth suppression by some inhibiting factors, such as total suspended solids and organic nitrogen, limited biomass productivity, and substantial dilution (5-10% v/v FWL) was required. To alleviate this suppression, anaerobic bacterial digestion was proposed to pretreat FWL and convert certain nutrients such as organic nitrogen to ammonium. The pretreatment was optimized in neutral to slightly alkaline media, where a byproduct of biomethane up to 4.67 L methane/kg COD was produced. In addition, digestate after anaerobic ammonification can provide sufficient inorganic nutrients for subsequent microalgal biofuel production. Through batch cultivation, 50% (v/v) of anaerobic bacterial pretreated FWL digestate can be fed to D. tertiolecta, with biomass productivity of up to 0.88 g/L/day, and biomass productivity can be increased to 0.34 g/L/day for C. aponinum at 30% FWL digestate. Regarding the nutrient removal efficiency, 98.99% of total nitrogen and 65% of total phosphorus can be removed by D. tertiolecta, whereas more than 80% of total nitrogen and 65% of total phosphorus can be removed by C. aponinum. The use of anaerobic bacterial ammonification pretreatment can significantly improve the performance of subsequent microalgal treatments and has been shown to be a sustainable green technology for biofuel production and FWL recycling.

Keywords: Algal lipids production; Anaerobic digestion; Cyanobacterium apononium; Dunaliella tertiolecta; Food waste leachate.