Abstract
Purpose:
Purpose The role of endothelial Yes-associated protein 1 (YAP) in the pathogenesis of retinal angiogenesis and the astrocyte network in the mouse oxygen-induced retinopathy (OIR) model is unknown.
Methods:
For in vivo studies, OIR was induced in conditional endothelial YAP knockout mice and their wild-type littermates. Retinal vascularization and the astrocyte network were evaluated by whole-mount fluorescence and Western blotting. In vitro experiments were performed in astrocytes cultured with human microvascular endothelial cell-1-conditioned medium to analyze the mechanisms underlying the effect of endothelial YAP on astrocytes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing / physiology*
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Animals
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Animals, Newborn
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Astrocytes / pathology*
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Blotting, Western
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Cell Cycle Proteins / physiology*
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Cell Proliferation
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Cells, Cultured
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Culture Media, Conditioned
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Cytoplasm / metabolism
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Disease Models, Animal
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Endothelial Cells / metabolism*
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Fluorescent Antibody Technique, Indirect
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Leukemia Inhibitory Factor / metabolism*
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Mice, Transgenic
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Oxygen / toxicity
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Retinal Neovascularization / chemically induced
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Retinal Neovascularization / metabolism*
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Retinal Neovascularization / pathology
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Retinal Vessels / cytology
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Retinopathy of Prematurity / chemically induced
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Retinopathy of Prematurity / metabolism
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Retinopathy of Prematurity / pathology
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YAP-Signaling Proteins
Substances
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Adaptor Proteins, Signal Transducing
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Cell Cycle Proteins
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Culture Media, Conditioned
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Leukemia Inhibitory Factor
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Lif protein, mouse
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YAP-Signaling Proteins
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Yap1 protein, mouse
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Oxygen