3,4,5-Tri- O-Caffeoylquinic Acid Promoted Hair Pigmentation Through β-Catenin and Its Target Genes

Meriem Bejaoui; Myra O Villareal; Hiroko Isoda

doi:10.3389/fcell.2020.00175

3,4,5-Tri- O-Caffeoylquinic Acid Promoted Hair Pigmentation Through β-Catenin and Its Target Genes

Front Cell Dev Biol. 2020 Mar 25:8:175. doi: 10.3389/fcell.2020.00175. eCollection 2020.

Authors

Meriem Bejaoui¹, Myra O Villareal^{1

2

3}, Hiroko Isoda^{1

2

3}

Affiliations

¹ School of Integrative and Global Majors (SIGMA), University of Tsukuba, Tsukuba, Japan.
² Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan.
³ Alliance for Research on the Mediterranean and North Africa (ARENA), University of Tsukuba, Tsukuba, Japan.

Abstract

The hair follicle undergoes a regular cycle composed of three phases: anagen, catagen, and telogen. The life of follicular melanocytes is totally linked to the hair cycle; and during anagen or the growth phase, the melanocytes are active and produce the melanin responsible of hair shaft pigmentation. Various signaling pathways regulate the hair growth cycle and, therefore, the pigmentation; we distinguish the Wnt/β-catenin signaling pathway as it plays a major role in the development, growth, and proliferation of the melanocytes and the activation of melanogenesis enzymes and the related transcription factor. In this study, 3,4,5-tri-O-caffeoylquinic acid (TCQA), a caffeoylquinic acid derivative, stimulated the pigmentation in C3H mouse hair follicle, in human melanocytes, and B16F10 melanoma cells. An enhancement in pigmentation associated genes was observed upon TCQA treatment in vivo and in vitro. Interestingly, the expression of β-catenin was remarkably upregulated in mouse treated skin and in pigment cell lines. Moreover, TCQA upregulated CTNNB1 expression after inhibition in human melanocytes. Taken together, this study suggests that TCQA triggered β-catenin activation to enhance the pigmentation during the anagen phase of the hair cycle.

Keywords: 3; 4; 5-tri-O-caffeoylquinic acid; anagen; melanin; melanocytes; melanogenesis enzymes; microphthalmia-associated transcription factor; β-catenin.