An extracellular environment is critical in neuronal development and growth. Changes in neuronal morphology, neuron adhesion, and even the rate of neurite formation, can be modified by both the chemical and physical properties of interfacing substrates. Topography has a major impact on neuronal growth. Neuronal behavior and morphology are affected by the size, shape and pattern of the topographic elements. Combining topography with active materials may lead to enhanced influence. This paper demonstrates the effects of silver nanolines (AgNLs) on the growth pattern of SH-SY5Y cells. The morphology of the cells atop the nanotopographical substrates is measured, revealing a significant promoting effect. The number of neurites initiating from the soma is larger in SH-SY5Y cells plated on AgNLs than in control samples. The cells also exhibit an increase in neurite branching points towards more complex structures. These results indicate that substrates decorated with nanotopography affect cellular growth in a way that may be useful for enhanced regeneration, opening new possibilities for electrode and implant design.