In the last few years carbon nanostructures have been applied for the immobilization of enzymes and biomimetic organo-metallic species useful for biotechnological applications. The nature of the support and the method of immobilization are responsible for the stability, reactivity and selectivity of the system. In this review, we focus on the recent advances in the use of carbon nanostructures, carbon nanotubes, carbon nanorods, fullerene and graphene for the preparation of biocatalytic and biomimetic systems and for their application in the development of green chemical processes.