Preparation of graphene nano-sheet bonded PDA/MOF microcapsules with immobilized glucose oxidase as a mimetic multi-enzyme system for electrochemical sensing of glucose

Yang Wang; Chen Hou; Yun Zhang; Fu He; Mingzhu Liu; Xiaoli Li

doi:10.1039/c6tb00276e

Preparation of graphene nano-sheet bonded PDA/MOF microcapsules with immobilized glucose oxidase as a mimetic multi-enzyme system for electrochemical sensing of glucose

J Mater Chem B. 2016 Jun 7;4(21):3695-3702. doi: 10.1039/c6tb00276e. Epub 2016 May 3.

Authors

Yang Wang¹, Chen Hou, Yun Zhang, Fu He, Mingzhu Liu, Xiaoli Li

Affiliation

¹ State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, College of Resources and Environment, Institute of Biochemical Engineering & Environmental Technology, Gansu Key Laboratory for Environmental Pollution Prediction and Control, College of Earth and Environmental Sciences, Lanzhou University, Lanzhou 730000, China. zhangyun@lzu.edu.cn mzliu@lzu.edu.cn.

PMID: 32263308
DOI: 10.1039/c6tb00276e

Abstract

A novel amperometric biosensor for glucose detection was fabricated based on a mimetic multi-enzyme system by combing mimetic enzymes (metal-organic frameworks, MOFs) and glucose oxidase (GOx). The microcapsule constructed with polydopamine (PDA) and ZIF-8 using CaCO₃ templates could be utilized not only to provide the biocompatible and non-toxic microenvironment to immobilize GOx, but also as an efficient mimetic enzyme because of the peroxidase-like properties of ZIF-8. The graphene nano-sheets (rGO) were bonded to the PDA/ZIF-8 microcapsules to accelerate the electron transfer between the microcapsule and the electrode, and the resulted PDA/ZIF-8@rGO showed excellent electro-catalytic activity towards H₂O₂. The mimetic multi-enzyme system was fabricated when GOx was immobilized in the as-prepared microcapsule, and the developed GOx/PDA/ZIF-8@rGO/GCE biosensor exhibited extraordinary electro-detection performance for glucose with two wide linear ranges from 1 μM to 1.2 mM and 1.2 mM to 3.6 mM, and the limit of detection was 0.333 μM (S/N = 3). Moreover, the good selectivity for glucose and satisfactory detection results in real samples of the prepared biosensor were also demonstrated.