Degradable polyurethane (PU) with copolyester oligomer consisting of ε-caprolactone (CL) and glycolide (GA) as the soft segments has been prepared by a combination of ring-opening polymerization and condensation reaction. Enzymatic and hydrolytic degradation experiments demonstrate that the PU can degrade in seawater. Such a polyurethane exhibit a more rapid degradation in comparison with that with poly(ε-caprolactone) (PCL) soft segments because the introduction of GA can reduce the crystallinity, as revealed by differential scanning calorimetry (DSC) and polarizing optical microscope (POM). Marine field tests show that the degradable polyurethane has good antifouling ability due to its self-renewal property. Besides, such polyurethane can serve as a carrier and controlled release system for an antifoulant, and the incorporation of an antifoulant in the polyurethane can significantly improve the antifouling ability and duration.