INS-fOCT: a label-free, all-optical method for simultaneously manipulating and mapping brain function

Ying Zhang; Lin Yao; Fen Yang; Shanshan Yang; Akshay Edathodathil; Wang Xi; Anna Wang Roe; Peng Li

doi:10.1117/1.NPh.7.1.015014

INS-fOCT: a label-free, all-optical method for simultaneously manipulating and mapping brain function

Neurophotonics. 2020 Jan;7(1):015014. doi: 10.1117/1.NPh.7.1.015014. Epub 2020 Mar 31.

Authors

Ying Zhang^{1

2}, Lin Yao¹, Fen Yang², Shanshan Yang¹, Akshay Edathodathil², Wang Xi², Anna Wang Roe^{2

3

4}, Peng Li^{1

5}

Affiliations

¹ Zhejiang University, College of Optical Science and Engineering, State Key Lab of Modern Optical Instrumentation, Hangzhou, China.
² Zhejiang University, Interdisciplinary Institute of Neuroscience and Technology, School of Medicine, Hangzhou, Zhejiang, China.
³ Zhejiang University, Key Laboratory of Biomedical Engineering of Ministry of Education, Hangzhou, Zhejiang, China.
⁴ Oregon Health & Sciences University, Oregon National Primate Research Center, Division of Neuroscience, Beaverton, Oregon, United States.
⁵ Zhejiang University, International Research Center for Advanced Photonics, Hangzhou, Zhejiang, China.

Abstract

Significance: Current approaches to stimulating and recording from the brain have combined electrical or optogenetic stimulation with recording approaches, such as two-photon, electrophysiology (EP), and optical intrinsic signal imaging (OISI). However, we lack a label-free, all-optical approach with high spatial and temporal resolution. Aim: To develop a label-free, all-optical method that simultaneously manipulates and images brain function using pulsed near-infrared light (INS) and functional optical coherence tomography (fOCT), respectively. Approach: We built a coregistered INS, fOCT, and OISI system. OISI and EP recordings were employed to validate the fOCT signals. Results: The fOCT signal was reliable and regional, and the area of fOCT signal corresponded with the INS-activated region. The fOCT signal was in synchrony with the INS onset time with a delay of $\sim 30 ms$ . The magnitude of fOCT signal exhibited a linear correlation with the INS radiant exposure. The significant correlation between the fOCT signal and INS was further supported by OISI and EP recordings. Conclusions: The proposed fiber-based, all-optical INS-fOCT method allows simultaneous stimulation and mapping without the risk of interchannel cross-talk and the requirement of contrast injection and viral transfection and offers a deep penetration depth and high resolution.

Keywords: functional imaging; functional optical coherence tomography; infrared neural stimulation.

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