MicroRNA-122-5p inhibits cell proliferation, migration and invasion by targeting CCNG1 in pancreatic ductal adenocarcinoma

Chen Dai; Yan Zhang; Zhihua Xu; Mengxian Jin

doi:10.1186/s12935-020-01185-z

MicroRNA-122-5p inhibits cell proliferation, migration and invasion by targeting CCNG1 in pancreatic ductal adenocarcinoma

Cancer Cell Int. 2020 Mar 30:20:98. doi: 10.1186/s12935-020-01185-z. eCollection 2020.

Authors

Chen Dai^#¹, Yan Zhang^#², Zhihua Xu¹, Mengxian Jin³

Affiliations

¹ 2Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, 215006 China.
² 3Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou, 215006 China.
³ Department of Endocrinology, Suzhou Xiangcheng People's Hospital, Suzhou, 215131 China.

^# Contributed equally.

Abstract

Background: Pancreatic ductal adenocarcinoma (PDAC) is a lethal human malignancy, and previous researches support the contribution of microRNA (miRNA) to cancer progression. MiR-122-5p is reported to participate in the regulation of various cancers, while the function of miR-122-5p in PDAC remains unclear. In this study, we investigated the precise mechanism of miR-122-5p involved in PDAC pathogenesis.

Methods: The expression levels of miR-122-5p were detected in human PDAC tissues and cell lines by miRNA RT-PCR. The effects of miR-122-5p on cell proliferation were explored by MTT assays, colony formation assays and flow cytometry assays. The ability of migration and invasion was determined by transwell assays. Dual Luciferase reporter assay was performed to validate the direct interaction between miR-122-5p and its target gene. The related molecules of cell cycle, apoptosis and epithelial-mesenchymal transition (EMT) were examined with qRT-PCR and western blot. In addition, xenograft mouse models were applied to explore the effects of miR-122-5p in vivo.

Results: MiR-122-5p was underexpressed, while CCNG1 was highly expressed in PDAC tissues and cells. MiR-122-5p was negatively correlated with TNM stage, tumor size and lymph node metastasis in PDAC patients. Overexpression of miR-122-5p suppressed the proliferation, migration and invasion in vitro and inhibited tumorigenesis in vivo. Furthermore, CCNG1 was a direct target of miR-122-5p. Upregulated CCNG1 could partially reverse the effects caused by miR-122-5p. Moreover, miR-122-5p inhibited EMT through downregulation of CCNG1.

Conclusion: Overexpression of miR-122-5p could inhibit cell proliferation, migration, invasion, and EMT by downregulating CCNG1 in PDAC, suggesting a potential therapeutic target for PDAC.

Keywords: CCNG1; MiR-122-5p; PDAC.